Effect of microencapsulated cell preparation technology and conditions on the catalytic performance of Penicillium purpurogenum Li-3 strain cells
Effect of microencapsulated cell preparation technology and conditions on the catalytic performance of Penicillium purpurogenum Li-3 strain cells
复制标题
微囊化细胞制备工艺及条件对紫青霉Li-3株细胞催化性能的影响
DOI：
10.1016/j.procbio.2014.01.015
复制
发表时间：
2014-05-01
影响因子：
4.4
通讯作者：
Ouyang, Qiao-Feng
中科院分区：
文献类型：
--
作者：
Cao, Hong;Ye, Hai;Ouyang, Qiao-Feng
将微囊化细胞制备技术应用于产紫青霉Li-3全细胞催化水解甘草次酸（GL）制备生物利用度高、甜度高、安全性好的甘草次酸单葡萄糖醛酸苷（GAMG）。研究了海藻酸钠-壳聚糖微囊化产紫青霉Li-3菌株细胞的关键技术和工艺条件对其使用性能的影响。结果表明，工艺条件是微囊化细胞发挥最佳作用的关键。细胞微胶囊化后，传质效率略有下降，导致催化活性略有下降。然而，细胞在微囊化后获得有效的保护，因此表现出更好的生长。连续使用12个批次后，微囊化细胞的剩余活性为50.11%，微囊破损率仅为6.4%。因此，微囊化P. purpurogenumLi-3菌株细胞具有较高的机械强度和稳定性。(C)2014爱思唯尔有限公司版权所有。
Microencapsulated cell preparation technology was applied to the hydrolysis of glycyrrhizin (GL) with Penicillium purpurogenum Li-3 whole-cell catalytic technology into glycyrrhetinic acid monoglucuronide (GAMG) possessing better bioavailability, sweetness, and security. The effect of the key techniques and technological conditions used for preparation of alginate chitosan microencapsulated P. purpurogenum Li-3 strain cell on its usability was investigated. Results showed that technological conditions were crucial for microencapsulated cells to play the best. After cells were micro encapsulated, mass transfer efficiency decreased slightly, resulting in a small decrease in catalytic activity. However, the cells obtained valid protection following microencapsulation, and thus exhibited better growth. Moreover, after continuous use for 12 batch cycles, 50.11% residual activity of the microencapsulated cells remained, and the breakage rate of microcapsules was only 6.4%. Therefore, microencapsulated P. purpurogenum Li-3 strain cells possessed comparatively high mechanical strength and stability. (C) 2014 Elsevier Ltd. All rights reserved.