Structural and Functional NIR-II Fluorescence Bioimaging in Urinary System via Clinically Approved Dye Methylene Blue
Structural and Functional NIR-II Fluorescence Bioimaging in Urinary System via Clinically Approved Dye Methylene Blue
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DOI：
10.1101/2020.01.24.917955
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Dingwei Xue;Di Wu;Zeyi Lu;Abudureheman Zebibula;Zhe Feng;J. Qian;Gonghui Li
中科院分区：
文献类型：
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作者：
Dingwei Xue;Di Wu;Zeyi Lu;Abudureheman Zebibula;Zhe Feng;J. Qian;Gonghui Li
准确的结构和功能成像对泌尿系统疾病的诊断和预后至关重要。近红外区域（NIR）II荧光成像与传统成像方法相比具有高灵敏度、高安全性和快速反馈的优点，但其临床适用性有限。在此，我们首次报道了由临床批准的和肾可清除的NIR染料亚甲蓝实现的泌尿系统的体内NIR-II荧光成像，其不仅可以实现清晰的侵入性/非侵入性尿路造影，而且可以无创地检测肾功能。这些结果表明，MB辅助的NIR-II荧光成像具有很大的希望，临床上的泌尿系统的侵入性/非侵入性结构成像和临床前的动物模型中的肾功能的调查。
Accurate structural and functional imaging is vital for the diagnosis and prognosis of the urinary system diseases. Near-infrared region (NIR) II fluorescence imaging has shown advantages of high sensitivity, high safety, and fast feedback compared to the conventional imaging methods but limited to its clinical applicability. Herein, we first report that in vivo NIR-II fluorescence imaging of the urinary system enabled by clinically approved and renal-clearable NIR dye methylene blue, which can not only achieve clear invasive/non-invasive urography but also noninvasively detect renal function. These results demonstrate that MB assisted NIR-II fluorescence imaging holds great promise for invasive/noninvasive structural imaging of the urinary system clinically and investigation of renal function in animal models preclinically.