Proteomic changes associated with predator‐induced morphological defences in oysters
Proteomic changes associated with predator‐induced morphological defences in oysters
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DOI：
10.1111/mec.16580
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发表时间：
2022-06
影响因子：
4.9
通讯作者：
T. G. Evans;Jillian Bible;A. Maynard;Kaylee R. Griffith;E. Sanford;D. Kültz
中科院分区：
文献类型：
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作者：
T. G. Evans;Jillian Bible;A. Maynard;Kaylee R. Griffith;E. Sanford;D. Kültz
当生物体经历表型的可塑性变化以降低捕食风险时，诱导性猎物防御发生。当捕食压力在空间或时间上持续变化时，例如当捕食者和猎物仅在部分地理范围内共存时，猎物种群可以在其诱导防御中局部适应。在加州河口，本地奥林匹亚牡蛎（Ostrea lurida）种群已经进化出不同的表型反应的入侵捕食者，大西洋牡蛎钻（Urosalpinx cinerea）。在这项研究中，牡蛎从一个河口与钻，牡蛎从河口没有钻，饲养了两代人在实验室的共同花园，随后暴露于线索大西洋钻。比较蛋白质组学，然后被用来调查其诱导防御保守和不同方面的分子机制。这两个群体开发更小，更厚，更硬的壳钻暴露后，这些变化壳表型与上调钙转运蛋白，可能会影响生物矿化。诱导防御进化的部分原因是，当捕食风险较低时，防御表型会产生健身成本。免疫蛋白在暴露于钻头后被两个牡蛎种群下调，这意味着生物矿化和免疫功能之间的权衡。在钻头暴露后，与钻头共存的种群中的牡蛎比栖息在尚未被捕食者入侵的河口的牡蛎长出更小的壳。不同人群对钻孔的反应差异与同种型特异性蛋白表达相关。这一趋势表明，通过修改现有机制，牡蛎中进化出了更强的诱导防御反应，与钻头共同发生。
Inducible prey defences occur when organisms undergo plastic changes in phenotype to reduce predation risk. When predation pressure varies persistently over space or time, such as when predator and prey co‐occur over only part of their biogeographic ranges, prey populations can become locally adapted in their inducible defences. In California estuaries, native Olympia oyster (Ostrea lurida) populations have evolved disparate phenotypic responses to an invasive predator, the Atlantic oyster drill (Urosalpinx cinerea). In this study, oysters from an estuary with drills, and oysters from an estuary without drills, were reared for two generations in a laboratory common garden, and subsequently exposed to cues from Atlantic drills. Comparative proteomics was then used to investigate molecular mechanisms underlying conserved and divergent aspects of their inducible defences. Both populations developed smaller, thicker, and harder shells after drill exposure, and these changes in shell phenotype were associated with upregulation of calcium transport proteins that could influence biomineralization. Inducible defences evolve in part because defended phenotypes incur fitness costs when predation risk is low. Immune proteins were downregulated by both oyster populations after exposure to drills, implying a trade‐off between biomineralization and immune function. Following drill exposure, oysters from the population that co‐occurs with drills grew smaller shells than oysters inhabiting the estuary not yet invaded by the predator. Variation in the response to drills between populations was associated with isoform‐specific protein expression. This trend suggests that a stronger inducible defence response evolved in oysters that co‐occur with drills through modification of an existing mechanism.