Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
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DOI：
10.1002/rcm.1111
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发表时间：
2003-01-01
影响因子：
2
通讯作者：
Muddiman, DC
中科院分区：
文献类型：
--
作者：
Null, AP;Muddiman, DC
由于同位素丰度的自然分布引入的负偏差，通过质谱法对大分子的平均质量的实验测定受到限制。这导致了质心顶部（ToC）的测量，而不是真正的质心。我们已经开发了一种实用的校正因子，应用于ToC测量，以在很大程度上消除自然界引入的系统偏差。使用平均分子量（
The experimental determination of average mass by mass spectrometry is limited for large molecules due to the negative bias introduced by the natural distribution of isotopic abundances. This results in the measurement of the top-of-centroid (ToC) as opposed to the true centroid. We have developed a practical correction factor that is applied to the ToC measurement to largely remove the systematic bias introduced by nature. The correction factor is calculated easily using the average molecular mass (