Interactive Effects of Seawater Acidification and Elevated Temperature on the Transcriptome and Biomineralization in the Pearl Oyster Pinctada fucata
Interactive Effects of Seawater Acidification and Elevated Temperature on the Transcriptome and Biomineralization in the Pearl Oyster Pinctada fucata
复制标题
海水酸化和升高温度对珠母贝转录组和生物矿化的相互作用影响
DOI：
10.1021/acs.est.5b05107
复制
发表时间：
2016-02-02
影响因子：
11.4
通讯作者：
Zhang, Rongqing
中科院分区：
文献类型：
--
作者：
Li, Shiguo;Huang, Jingliang;Zhang, Rongqing
海洋酸化和海洋变暖对海洋钙化菌的交互作用因物种而异，但对其潜在机制知之甚少。本研究调查了海水酸化和温度升高的综合影响（环境条件：pH 8.1 × 23 ℃，胁迫条件：pH 7.8 × 23 ℃、pH 8.1 × 28 ℃和pH 7.8 × 28 ℃，暴露时间：两个月）对合浦珠母贝（Pinctada fucata）转录组和生物矿化的影响，合浦珠母贝是一种重要的海洋钙化剂。转录组分析表明，在海水酸化的单独作用下，合浦原螯虾通过补偿性酸碱机制、代谢抑制和积极的生理反应来减轻酸化的影响。这些反应是能源昂贵的过程，导致净钙化率，壳表面钙和碳含量的下降，并在壳的超微结构的变化。温度升高（28摄氏度）的温度窗口内的P. fucata没有诱导显着富集的测序基因，相反，促进钙化，这被检测到，以减轻负的生物矿化和壳的超微结构。总体而言，这项研究将有助于阐明珍珠牡蛎对海水条件变化的反应机制，并预测全球气候变化对珍珠养殖的影响。
Interactive effects of ocean acidification and ocean warming on marine calcifiers vary among species, but little is known about the underlying mechanisms. The present study investigated the combined effects of seawater acidification and elevated temperature (ambient condition: pH 8.1 X 23 degrees C, stress conditions: pH 7.8 X 23 degrees C, pH 8.1 x 28 degrees C, and pH 7.8 x 28 degrees C, exposure time: two months) on the transcriptome and biomineralization of the pearl oyster Pinctada fucata, which is an important marine calcifier. Transcriptome analyses indicated that P. fucata implemented a compensatory acid base mechanism, metabolic depression and positive physiological responses to mitigate the effects of seawater acidification alone. These responses were energy-expensive processes, leading to decreases in the net calcification rate, shell surface calcium and carbon content, and changes in the shell ultrastructure. Elevated temperature (28 degrees C) within the thermal window of P. fucata did not induce significant enrichment of the sequenced genes and conversely facilitated calcification, which was detected to alleviate the negative biomineralization and the shell ultrastructure. Overall, this study will help elucidate the mechanisms by which pearl oysters respond to changing seawater conditions and predict the effects of global climate change on pearl aquaculture.