Nucleotide sequence and transcription of the fbc operon from Rhodopseudomonas sphaeroides. Evaluation of the deduced amino acid sequences of the FeS protein, cytochrome b and cytochrome c1.
Nucleotide sequence and transcription of the fbc operon from Rhodopseudomonas sphaeroides. Evaluation of the deduced amino acid sequences of the FeS protein, cytochrome b and cytochrome c1.
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球形红假单胞菌 fbc 操纵子的核苷酸序列和转录。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
W. Sebald
中科院分区：
文献类型：
--
作者：
N. Gabellini;W. Sebald
来自球形红假单胞菌的 fbc 操纵子编码泛醇-细胞色素-c 还原酶（b/c1 复合物）的三个氧化还原载体：FeS 蛋白、细胞色素 b 和细胞色素 c1 [Gabellini, N. 等人。 （1985）EMBO J.2，549-553]。克隆的球形红藻染色体DNA的3874 bp的核苷酸序列已被确定，包括三个结构基因fbcF、fbcB和fbcC。由于fbc基因编码的氨基酸序列与相应线粒体多肽的序列高度同源，因此可以容易地鉴定fbc基因的阅读框。通过 S1 核酸酶保护分析研究了转录的起始点和终止点。 fbc操纵子的转录从fbcF基因起始密码子上游约240个碱基对处开始，并在fbcC基因终止密码子下游120个碱基对处终止。鉴定出类似于RNA聚合酶结合和释放的识别信号的核苷酸序列。通过对分离的亚基进行自动 Edman 降解获得成熟细胞色素 c1 的 N 端氨基酸序列，确认了 fbcC 阅读框并表明细菌前脱辅基细胞色素 c1 具有包含 21 个残基的瞬时前导序列。还获得了 FeS 蛋白的一种亲水肽的 N 端序列，证实了 fbcF 阅读框。讨论了与线粒体和叶绿体同源蛋白的已知一级结构相关的推导的氨基酸序列。多肽的一级结构根据其在膜中的拓扑结构、生物起源、催化位点的结构和亚基相互作用进行评估。
The fbc operon from Rhodopseudomonas sphaeroides encodes the three redox carriers of the ubiquinol-cytochrome-c reductase (b/c1 complex): FeS protein, cytochrome b and cytochrome c1 [Gabellini, N. et al. (1985) EMBO J.2, 549-553]. The nucleotide sequence of 3874 bp of cloned R. sphaeroides chromosomal DNA, including the three structural genes fbcF, fbcB and fbcC has been determined. The reading frames of the fbc genes could be identified readily since the encoded amino acid sequences are highly homologous with the sequences of the corresponding mitochondrial polypeptides. Initiation and termination points for transcription have been investigated by S1 nuclease protection analysis. The transcription of the fbc operon starts approximately 240 base pairs upstream from the start codon of the fbcF gene and terminates 120 base pairs downstream from the stop codon of the fbcC gene. Nucleotide sequences resembling recognition signals for the binding and release of the RNA polymerase were identified. The N-terminal amino acid sequence of the mature cytochrome c1 was obtained by automated Edman degradation of the isolated subunit, confirming the fbcC reading frame and indicating that the bacterial preapocytochrome c1 has a transient leader sequence including 21 residues. The N-terminal sequence of one hydrophilic peptide of the FeS protein has been also obtained confirming the fbcF reading frame. The deduced amino acid sequences are discussed in relation to the known primary structures of the homologous proteins from mitochondria and chloroplasts. The primary structures of the polypeptides are evaluated with respect to their topology in the membrane, their biogenesis, the structure of the catalytic sites and subunit interactions.
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Nobrega,FG;Tzagoloff,A
通讯作者：
Tzagoloff,A
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S
泛醌结合蛋白。
DOI：
10.1016/0304-4173(81)90007-0
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Yu,CA;Yu,L
通讯作者：
Yu,L