Gut microbiota composition is correlated to grid floor induced stress and behavior in the BALB/c mouse.
Gut microbiota composition is correlated to grid floor induced stress and behavior in the BALB/c mouse.
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DOI：
10.1371/journal.pone.0046231
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hansen AK
中科院分区：
文献类型：
--
作者：
Bangsgaard Bendtsen KM;Krych L;Sørensen DB;Pang W;Nielsen DS;Josefsen K;Hansen LH;Sørensen SJ;Hansen AK
应激对动物的胃肠道、免疫系统和行为有着深远的影响。在这项研究中，通过变性级凝胶电泳（DGGE）和标签编码的16 S rRNA基因扩增子焦磷酸测序（454/FLX）确定的肠道微生物群组成与三联测试（高架加迷宫，光/暗箱，和开放领域的组合），尾部悬挂测试，并在28只雌性BALB/c小鼠暴露于两周的网格地板诱导的压力挖掘行为之间的相关性进行了研究。细胞因子和葡萄糖水平在基线时，期间和暴露于网格地板后进行测量。通过DGGE和焦磷酸测序确定，对小鼠进行应激明显改变了盲肠微生物群。气味杆菌，Alistipes和一个未分类的属从Coriobacteriaceae家庭显着增加，在网格地板圈养的小鼠。与基线相比，暴露于网格地板住房的小鼠改变了在高架加迷宫、明/暗箱中花费的时间量和挖洞行为。网格地板饲养的小鼠在尾部悬挂测试中具有显著更长的不动持续时间，并且从基线增加了它们的不动发作次数。GM组成与IL-1α、IFN-γ、高架迷宫闭臂进入次数、高架迷宫总时间、明箱/暗箱时间、旷场内区时间及旷场总时间呈显著相关。发现与厚壁菌门（例如瘤胃球菌科和毛螺菌科的各种物种）的水平显著相关。除了从基线到结束水平总体下降外，未发现所评价的细胞因子的显著差异。在网格地板饲养的小鼠中发现血糖水平显著较低，而HbA 1c水平显著较高。它的结论是，网格地板住房改变GM的组成，这似乎影响某些焦虑相关的参数。
Stress has profound influence on the gastro-intestinal tract, the immune system and the behavior of the animal. In this study, the correlation between gut microbiota composition determined by Denaturing Grade Gel Electrophoresis (DGGE) and tag-encoded 16S rRNA gene amplicon pyrosequencing (454/FLX) and behavior in the Tripletest (Elevated Plus Maze, Light/Dark Box, and Open Field combined), the Tail Suspension Test, and Burrowing in 28 female BALB/c mice exposed to two weeks of grid floor induced stress was investigated. Cytokine and glucose levels were measured at baseline, during and after exposure to grid floor. Stressing the mice clearly changed the cecal microbiota as determined by both DGGE and pyrosequencing. Odoribacter, Alistipes and an unclassified genus from the Coriobacteriaceae family increased significantly in the grid floor housed mice. Compared to baseline, the mice exposed to grid floor housing changed the amount of time spent in the Elevated Plus Maze, in the Light/Dark Box, and burrowing behavior. The grid floor housed mice had significantly longer immobility duration in the Tail Suspension Test and increased their number of immobility episodes from baseline. Significant correlations were found between GM composition and IL-1α, IFN-γ, closed arm entries of Elevated Plus Maze, total time in Elevated Plus Maze, time spent in Light/Dark Box, and time spent in the inner zone of the Open Field as well as total time in the Open Field. Significant correlations were found to the levels of Firmicutes, e.g. various species of Ruminococccaceae and Lachnospiraceae. No significant difference was found for the evaluated cytokines, except an overall decrease in levels from baseline to end. A significant lower level of blood glucose was found in the grid floor housed mice, whereas the HbA1c level was significantly higher. It is concluded that grid floor housing changes the GM composition, which seems to influence certain anxiety-related parameters.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
2.7
作者：
Deacon, R. M. J.
通讯作者：
Deacon, R. M. J.
影响因子：
5.7
作者：
Fujimura KE;Slusher NA;Cabana MD;Lynch SV
通讯作者：
Lynch SV
DOI：
10.1152/ajpgi.00190.2011
发表时间：
2012-01-01
影响因子：
4.5
作者：
Bajaj, Jasmohan S.;Ridlon, Jason M.;Gillevet, Patrick M.
通讯作者：
Gillevet, Patrick M.
影响因子：
2.6
作者：
Dantzer, Robert
通讯作者：
Dantzer, Robert