SYNTHESIS OF NUCLEIC ACIDS AT INFECTION SITES OF SOYBEAN ROOTS PARASITIZED BY HETERODERA-GLYCINES
SYNTHESIS OF NUCLEIC ACIDS AT INFECTION SITES OF SOYBEAN ROOTS PARASITIZED BY HETERODERA-GLYCINES
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DOI：
10.1094/phyto-61-395
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发表时间：
1971-01-01
期刊：
影响因子：
3.2
通讯作者：
ENDO, BY
中科院分区：
文献类型：
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作者：
ENDO, BY
大豆孢囊线虫在大豆根中诱导的合胞体的特征是在一团致密的细胞质中存在大量膨大的核。在线虫发育的各个阶段，对受感染的根切片的观察表明，合胞体核在脱氧核糖核酸（DNA）合成方面相对静止。然而，氚标记的胸苷被纳入细胞核的合胞体位于皮层和血管区域的感染根。在发育中的合胞体中，DNA合成的主要位点位于合胞体的前缘。在这个过渡区的细胞核显示高 * H-甲基胸苷摄取，标志着活跃的DNA合成的网站。Phytopathology 61：395- 399.大豆胞囊线虫（Heterodera glycines）诱导的合胞体中核酸合成的定位尚未报道。在异囊线虫科的相关根结线虫属中，研究表明，由根结线虫诱导的合胞体引起明显的核变化，这可能与DNA和RNA的合成相关（14）。已经确定，由根结线虫属物种诱导的合胞体含有在不同扩大阶段经历有丝分裂的核（9）。不完全的有丝分裂没有胞质分裂可以产生多倍体细胞描述了不同的工作者（2，7，9，12）。除了通过染色体计数（7）直接证明多倍性外，还使用标记的DNA和RNA前体来证明这些核酸的合成位点（14）。通过使用DNA和RNA的特异性染色剂提供了核酸水平增加的进一步证据（1）。
Syncytia induced in soybean roots by the soybean cyst nematode are characterized by the presence of numerous enlarged nuclei in a mass of dense cyto-plasm. Observations of infected root sections made during various stages of nematode development show that syncytial nuclei are relatively quiescent in terms of deoxyribonucleic acid (DNA) synthesis. However, tritiated thymidine was incorporated in nuclei of syncytia located in both the cortical and vascular regions of infected roots. A primary site of DNA synthesis in a developing syncytium was at the leading edge of the syncytium. Nuclei of cells in this transitional zone showed high* H-methylthymidine uptake, signifying sites of active DNA synthesis. Phytopathology 61: 395-399.The localization of nucleic acid synthesis in syncytia induced by Heterodera glycines has not been reported. In the related Meloidogyne genus of the family Heteroderidae, studies have shown that syncytia induced by the root knot nematode cause obvious nuclear changes that can be correlated with the synthesis of DNA and RNA (14). It is well established that syncytia induced by species of Meloidogyne contain nuclei that undergo karyokinesis at various stages of enlargement (9). Incomplete karyokinesis without cytokinesis can give rise to the polyploid cells described by various workers (2, 7, 9, 12). In addition to direct evidence of polyploidy through chromosome counts (7), labeled precursors of DNA and RNA were used to demonstrate sites of synthesis of these nucleic acids (14). Further evidence for an increase in the levels of nucleic acids was provided through the use of specific stains for DNA and RNA (1).