immunostimulatory activity of aqueous extract isolated from Prunella vulgaris
immunostimulatory activity of aqueous extract isolated from Prunella vulgaris
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DOI：
10.1016/j.fct.2008.10.010
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Jeong, Hye Gwang
中科院分区：
文献类型：
--
作者：
Han, Eun Hee;Choi, Jae Ho;Jeong, Hye Gwang
夏枯草具有抗氧化和抗菌活性，是治疗疱疹性角膜炎的传统中药。在这项研究中，我们研究了普通疟原虫在小鼠巨噬细胞RAW 264.7细胞中的免疫刺激和抗肿瘤活性。因此，我们研究了一种水提取物的普通（PVAE）对巨噬细胞功能的影响。我们发现PVAE刺激巨噬细胞吞噬活性。一氧化氮（NO）产生和细胞抑制活性。此外，PVAE诱导基因表达和巨噬细胞相关细胞因子（如TNF-α、IL-1 β和IL-6）的产生。瞬时转染显示NF-κ B介导PVAE诱导的巨噬细胞相关细胞因子表达水平的增加。PVAE诱导的NF-κ B活化也显著激活了丝裂原活化蛋白激酶（MAP激酶）。NF-κ B抑制剂和MAP激酶抑制剂预处理可抑制PVAE诱导的NO生成和吞噬活性。这表明PVAE通过NF-κ B反式激活和MAP激酶激活刺激巨噬细胞激活。(C)2008爱思唯尔有限公司保留所有权利。
Prunella vulgaris (P. vulgaris) has been used as a traditional medicine in the clinical treatment of herpetic keratitis and for its antioxidative and antimicrobial activities. In this study, we examined the immunostimulatory and antitumor activity of P. vulgaris in murine macrophage RAW 264.7 cells. Thus, we investigated the effects of an aqueous extract of A vulgaris (PVAE) on macrophage function. We found that PVAE stimulated macrophage phagocytic activity. nitric oxide (NO) production and cytostatic activity. In addition, PVAE induced gene expression and production of macrophage-related cytokines such as TNF-alpha, IL-1 beta and IL-6. Transient transfection revealed that NF-kappa B mediated the PVAE-induced increases in macrophage-related cytokine expression levels. Mitogen-activated protein kinases (MAP Kinase) were also significantly activated by the PVAE-induced NF-kappa B activation. Pretreatment with NF-kappa B inhibitor and MAP Kinase inhibitors inhibited the NO production and the phagocytic activity induced by PVAE. This demonstrates that PVAE stimulates macrophage activation via NF-kappa B transactivation and MAP kinase activation. (C) 2008 Elsevier Ltd. All rights reserved.