Galectin-3 modulates ureteric bud branching in organ culture of the developing mouse kidney
Galectin-3 modulates ureteric bud branching in organ culture of the developing mouse kidney
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DOI：
10.1681/asn.v123515
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发表时间：
2001-03-01
影响因子：
13.6
通讯作者：
Woolf, AS
中科院分区：
文献类型：
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作者：
Bullock, SL;Johnson, TM;Woolf, AS
Galectin-3是一种哺乳动物β-半乳糖苷特异性凝集素，具有细胞生长、粘附和肿瘤转化功能。在人类表达模式的基础上，提出半乳糖凝集素-3调节胎儿集合管生长。本文提供的证据表明，半乳糖凝集素3可以调节小鼠输尿管芽/集合管谱系的分支形态发生。使用免疫组织化学，半乳糖凝集素-3未检测到早期后肾，但上调后，在胎儿肾成熟时，蛋白质是突出的髓质集合管的基底域。此外，半乳糖凝集素-3胚胎第11天和第12天的整个后肾文化抑制输尿管芽分支，而半乳糖凝集素-1并没有扰乱形态发生，也没有半乳糖凝集素-3突变缺乏野生型高亲和力结合扩展寡糖。外源性半乳糖凝集素-3延缓了整个后肾外植体中肾间充质向肾单位的转化，但不影响脊髓在离体肾间充质中诱导肾单位。最后，添加半乳糖凝集素-3的阻断抗血清引起胚胎第12天后肾培养1周的发育上皮细胞扩张和变形。半乳糖凝集素-3蛋白在肾脏成熟过程中的上调，主要是在它可以介导细胞/基质相互作用的位点，似乎调节输尿管树的生长。
Galectin-3 is a mammalian beta -galactoside-specific lectin with functions in cell growth, adhesion, and neoplastic transformation. On the basis of expression patterns in humans, it is proposed that galectin-3 modulates fetal collecting duct growth. This article provides evidence that galectin3 can modulate branching morphogenesis of the mouse ureteric bud/ collecting duct lineage. With the use of immunohistochemistry, galectin-3 was not detected in early metanephrogenesis but was upregulated later in fetal kidney maturation when the protein was prominent in basal domains of medullary collecting ducts. Addition of galectin-3 to embryonic days 11 and 12 whole metanephric cultures inhibited ureteric bud branching, whereas galectin-1 did not perturb morphogenesis, nor did a galectin-3 mutant lacking wild-type high-affinity binding to extended oligosaccharides. Exogenous galectin-3 retarded conversion of renal mesenchyme to nephrons in whole metanephric explants but did not affect nephron induction by spinal cord in isolated renal mesenchymes. Finally, addition of a blocking antiserum to galectin-3 caused dilation and distortion of developing epithelia in embryonic day 12 metanephroi cultured for 1 wk. The upregulation of galectin-3 protein during kidney maturation, predominantly at sites where it could mediate cell/matrix interactions, seems to modulate growth of the ureteric tree.