Transdermal Electrochemical Monitoring of Glucose via High-Density Silicon Microneedle Array Patch
Transdermal Electrochemical Monitoring of Glucose via High-Density Silicon Microneedle Array Patch
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DOI：
10.1002/adfm.202009850
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发表时间：
2021-03-04
影响因子：
19
通讯作者：
Voelcker, Nicolas H.
中科院分区：
文献类型：
--
作者：
Dervisevic, Muamer;Alba, Maria;Voelcker, Nicolas H.
目前的血糖监测技术主要是用刺针穿刺取一小滴血，通过手持式血糖仪测量，不利于连续测量。间质液（ISF）作为一种可替代的生物体液正受到越来越多的关注。它的生化成分与血液非常相似，可以通过微创方法进行持续监测，这种方法不会引起疼痛，并将任何感染风险降至最低。本文提出了一种基于微针阵列（MNA）的透皮传感系统，用于无痛监测ISF葡萄糖。高密度硅微针（约9500微针cm(-2)）用于制备葡萄糖电化学监测的三电极贴片。MNA葡萄糖贴片在人工ISF中具有很好的选择性，灵敏度为0.1622 μ a mm(-1) cm(-2)，检出限为0.66 mm。微针阵列在小鼠体内的应用表明，MNA传感器获得的ISF葡萄糖浓度与商用血糖仪测定的血糖水平具有很好的相关性。因此，这种基于微针的传感系统为侵入性现有技术提供了另一种透皮诊断工具。
Current technology for blood glucose level monitoring is mainly based on the invasive finger-prick extraction of a small drop of blood using a lancet and measured via a handheld glucometer, which is not conducive to continuous measurements. Interstitial fluid (ISF) is gaining attention as an alternative biofluid. Its biochemical composition is very similar to that of blood and it can be monitored in a continuous manner via minimally invasive methods that cause no pain and minimize any risk of infection. Herein, a microneedle array (MNA) based transdermal sensing system for the pain free monitoring of ISF glucose is presented. High-density silicon microneedles (approximate to 9500 microneedles cm(-2)) are used to prepare a three-electrode patch for the electrochemical monitoring of glucose. The MNA glucose patch shows very good selectivity when tested in artificial ISF, with a sensitivity of 0.1622 mu A mm(-1) cm(-2) and a detection limit of 0.66 mm. In vivo application of the microneedle array in mice shows that the ISF glucose concentrations obtained with the MNA sensor gave very good correlation with the blood glucose levels determined with a commercial glucometer. This microneedle-based sensing system hence provides an alternative transdermal diagnostic tool to the invasive existing techniques.