Metabolic Effects of Angulation, Compression, and Reduced Mobility on Annulus Fibrosis in a Model of Altered Mechanical Environment in Scoliosis.
Metabolic Effects of Angulation, Compression, and Reduced Mobility on Annulus Fibrosis in a Model of Altered Mechanical Environment in Scoliosis.
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DOI：
10.1016/j.jspd.2013.02.001
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发表时间：
2013-05
期刊：
影响因子：
1.6
通讯作者：
Roughley, Peter J
中科院分区：
文献类型：
--
作者：
Stokes, Ian A F;McBride, Carole A;Aronsson, David D;Roughley, Peter J
不同力学条件下六组大鼠尾部椎间盘组织的比较。利用产生椎间盘狭窄和楔入的未成熟大鼠模型，确定与脊柱侧凸畸形的生物力学环境改变相关的椎间盘环改变。椎间盘在脊柱侧凸曲线中变得楔形和狭窄，可能部分是由于生物力学环境的改变。5周龄未成熟的Sprague-Dawley大鼠尾盘在一个改变机械环境的外部装置上进行了5周的加载和变形排列。四组大鼠(A)成角15度，(B)成角0.1 MPa压缩，(C)成角0.1 MPa压缩，(R)活动能力降低，并设假手术组和对照组。5周后测定叶片高度变化和基质组成（水分、DNA、GAG和HA含量），5天和5周分别测定脯氨酸和硫酸盐掺入量和mRNA表达量。5周后，与内部对照相比，所有四个干预组的椎间盘间隙明显缩小。相对于内部对照，水含量和细胞（DNA含量）在干预水平上没有差异，在成角椎间盘的凹面和凸面之间没有差异。压缩组织中（B组和C组）GAG含量增加，与预期一致，压缩导致透明质酸大小减少。在成角椎间盘和压缩椎间盘的凹侧观察到轻微增加的氚化脯氨酸掺入。A组和B组基因表达的不对称，以及一些组间差异，并没有发现椎间盘对机械改变反应的一致模式。该模型的椎间盘在5周后出现明显变窄，组织组成变化很小，代谢改变的证据也很少。
Comparison of disc tissue from rat tails in six groups having different mechanical conditions imposed. To identify disc annulus changes associated with the supposed altered biomechanical environment in a spine with scoliosis deformity using an immature rat model that produces disc narrowing and wedging. Intervertebral discs become wedged and narrowed in a scoliosis curve, probably due in part to altered biomechanical environment. Tail discs of 5-week-old immature Sprague-Dawley rats were subjected to an altered mechanical environment using an external apparatus applying permutations of loading and deformity for 5 weeks. Four groups of rats (A) 15 degrees Angulation, (B) Angulation with 0.1 MPa Compression, (C) 0.1 MPa Compression, and (R) Reduced mobility, together with a sham and a control group were studied. Disc height changes and matrix composition (water, DNA, GAG and HA content) were measured after 5 weeks, and proline and sulphate incorporation and mRNA expression were measured at 5 days and 5 weeks. After 5 weeks, disc space was significantly narrowed relative to internal controls in all four intervention groups. Water content and cellularity (DNA content) were not different at interventional levels relative to internal controls and not different between the concave and convex sides of the angulated discs. There was increased GAG content in compressed tissue (in Groups B and C), as expected, and compression resulted in a decrease in hyaluronic acid size. Slightly increased incorporation of tritiated-proline into the concave side of angulated discs and compressed discs was observed. Asymmetries of gene expression in Groups A and B, and some group-wise differences, did not identify consistent patterns associating the discs’ responses to mechanical alterations. Intervertebral discs in this model underwent substantial narrowing after 5 weeks, with minimal alteration in tissue composition and minimal evidence of metabolic changes.