TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD
TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD
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DOI：
10.1016/j.bbrc.2004.06.140
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发表时间：
2004-08-20
影响因子：
3.1
通讯作者：
Sakamoto, H
中科院分区：
文献类型：
--
作者：
Saito, K;Fujiwara, T;Sakamoto, H
Hu蛋白是rna结合蛋白，参与控制稳定、核输出和/或在3'-非翻译区具有富au元件（AREs）的特定mrna的翻译。三种神经元特异性的Hu蛋白（HuD， HuB和HuC），而不是普遍表达的Hu蛋白HuR，在大鼠神经元细胞系PC12中过表达时具有诱导神经突生长的活性。我们发现TAP/NXF1是大量mRNA的主要输出受体，是HuD的特定结合伙伴。利用重组蛋白进行的体外结合实验表明，TAP与HuD之间的相互作用是直接的，HuD可以与TAP和RNA形成三元配合物。有趣的是，HuR不与TAP相互作用。这些结果表明，HuD作为一种新的接头蛋白，招募TAP，以有效地输出神经元细胞中含有are的mrna。(C) 2004爱思唯尔公司版权所有。
Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells. (C) 2004 Elsevier Inc. All rights reserved.