SAGE analysis of early oogenesis in the silkworm, Bombyx mori
SAGE analysis of early oogenesis in the silkworm, Bombyx mori
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DOI：
10.1016/j.ibmb.2006.11.001
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Shimada, Toru
中科院分区：
文献类型：
--
作者：
Funaguma, Shunsuke;Hashimoto, Shin-ichi;Shimada, Toru
为了鉴定与胚胎成囊细胞分化有关的基因，我们构建了两个3'长序列基因表达分析（Long SAGE）文库，分别来自1-3期和2-3期卵室，并比较了它们的基因表达谱。在这两个文库中，最常见的标签来源于相同的新转录本。该转录物不具有任何能够编码长度超过100个氨基酸的蛋白质的开放阅读框。RNA印迹分析表明，该转录本特异性和丰富的表达在卵巢，主要是生殖细胞的卵巢管。这些结果表明，卵子发生可能是由一个以前未确定的非编码RNA调控。阶段1-3和阶段2-3卵室文库之间的基因表达谱的比较揭示，272个标签在阶段1-3卵室中比在文库2中显著更丰富（p < 0.05并且至少两倍变化）。在差异表达的转录本中，对应于ATP合酶亚基d（3.1倍富集）和ATP合酶偶联因子6（9.1倍富集）的序列，表明它们参与囊细胞的细胞周期调节。(c)2006爱思唯尔有限公司保留所有权利。
To identify genes involved in the differentiation of Bombyx cystoblast, we constructed two 3' long serial analysis, of gene expression (Long SAGE) libraries from stage 1-3 or stage 2-3 egg chambers and compared their gene expression profiles. In both libraries, the most frequent tags were derived from the same novel transcript. The transcript does not have any open reading frame capable of encoding a protein with over 100 amino acids in length. RNA blot analysis revealed that this transcript is specifically and abundantly expressed in the Bombyx ovary, mainly the germ line cells in the ovarioles. These results suggest that Bombyx oogenesis may be regulated by a previously unidentified non-coding RNA. Comparison of the gene expression profiles between the stage 1-3 and stage 2-3 egg chamber libraries revealed that 272 tags were significantly more abundant in stage 1-3 egg chambers (p < 0.05 and at least two-fold change) than in library 2. Among the differentially expressed transcripts were the sequences that correspond to ATP synthase subunit d (3.1-fold enriched) and ATP synthase coupling factor 6 (9.1-fold enriched), suggesting that they are involved in regulation of cell cycle of cystocytes. (c) 2006 Elsevier Ltd. All rights reserved.