The impact of rhubarb polysaccharides on Toll-like receptor 4-mediated activation of macrophages.
The impact of rhubarb polysaccharides on Toll-like receptor 4-mediated activation of macrophages.
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DOI：
10.1016/j.intimp.2013.10.015
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发表时间：
2013-12
影响因子：
5.6
通讯作者：
Xiaolong Zhang;Jing Wang;Zhuozai Xu;Zhongqiu Li;Shilan Feng;Hong Lu
中科院分区：
文献类型：
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作者：
Xiaolong Zhang;Jing Wang;Zhuozai Xu;Zhongqiu Li;Shilan Feng;Hong Lu
Toll样受体4介导的急性前葡萄膜炎是一种严重的眼部炎症性疾病，新的靶向药物治疗是其主要治疗手段。本研究旨在探讨大黄多糖激活巨噬细胞的分子机制，为治疗TLR 4介导的急性前葡萄膜炎寻找新的治疗方法。采用免疫荧光法检测大黄多糖对细胞的激活状态，ELISA法检测细胞上清液中炎性细胞因子IL-1β、IFN-γ、IL-6和TNF-α的产生;采用Western blot和真实的time RT-PCR检测RP对TLR 4、MyD 88、NF-κB p65蛋白和基因表达的影响。ELISA、免疫荧光等方法检测大黄多糖对巨噬细胞的激活作用，并诱导TLR 4、MyD 88、NF-κB B p65蛋白和基因的表达，同时诱导细胞因子IL-1β、IFN-γ、IL-6和TNF-α的产生。结论大黄多糖通过TLR 4/NF-ΚB B信号通路激活巨噬细胞。我们的数据表明，RP有可能被用于其免疫刺激作用在感染性疾病，如TLR 4介导的急性前葡萄膜炎的治疗。
ObjectiveToll-like receptor 4-mediated acute anterior uveitis is a serious ocular inflammatory disease, and new targeted drug therapy is the primary treatment. The objective of the present study was to investigate the molecular mechanism responsible for the activation of macrophages by rhubarb polysaccharides (RP) and try to find a new treatment for TLR4 pathway-mediated acute anterior uveitis.MethodRAW264.7 macrophage cell lines were cultured in vitro, and rhubarb polysaccharides were used to stimulate the cell lines. An immunofluorescence technique was used to determine cell activation status by rhubarb polysaccharides; the ELISA technique was used to examine the production of cell supernatant inflammatory cytokines IL-1β, IFN-γ, IL-6, and TNF-α; and Western blot and real time RT-PCR were used to determine the effect of RP on TLR4, MyD88, and NF-κB p65 proteins and gene expression.ResultsUsing Western blot, real time RT-PCR, ELISA, and immunofluorescence techniques, we found that rhubarb polysaccharides in vitro could activate macrophages; induce TLR4, MyD88, and NF-κB p65 protein and gene expression; and also induce the production of cytokines IL-1β, IFN-γ, IL-6, and TNF-α.ConclusionThe results presented in this report suggest that RP induce macrophage activation through the TLR4/NF-ΚB signaling pathway. Our data suggest that RP have the potential to be used for their immunostimulatory effect in the treatment of infectious diseases such as TLR4-mediated acute anterior uveitis.