Removal of Foodborne Pathogen Biofilms by Acidic Electrolyzed Water.
Removal of Foodborne Pathogen Biofilms by Acidic Electrolyzed Water.
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酸性电解水去除食源性病原体生物膜
DOI：
10.3389/fmicb.2017.00988
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Han Q;Song X;Zhang Z;Fu J;Wang X;Malakar PK;Liu H;Pan Y;Zhao Y
生物膜是嵌入保护性细胞外聚合物 (EPS) 中的复杂微生物群落，在食品生产设施中很难去除。在这项研究中，评估了使用酸性电解水（AEW）去除食源性病原体生物膜的方法。我们使用绿色荧光蛋白标记的大肠杆菌来监测AEW去除生物膜的效率，在最佳处理条件下，生物膜中细胞的荧光信号迅速消失，生物膜细胞数量减少了67%以上。此外，AEW 引发 EPS 破坏，碳水化合物 C-O-C 键的变形以及氨基酸酪氨酸和苯丙氨酸中芳香环的变形表明了这一点。这些变形是通过 EPS 化学分析和拉曼光谱分析来识别的。扫描电子显微镜（SEM）图像证实，AEW 处理后生物膜的破裂和脱离得到增强。此外，AEW 还根除由革兰氏阴性菌（副溶血弧菌）和革兰氏阳性菌（单核细胞增生李斯特氏菌）形成的生物膜，并观察到使脱落的细胞失活，而脱落的细胞是潜在的二次污染源。这项研究表明，AEW 可能是一种可靠的食源性病原体生物膜破坏剂，也是食品行业传统使用的消毒剂的环保替代品。
Biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (EPS), are difficult to remove in food production facilities. In this study, the use of acidic electrolyzed water (AEW) to remove foodborne pathogen biofilms was evaluated. We used a green fluorescent protein-tagged Escherichia coli for monitoring the efficiency of AEW for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm disappeared rapidly and the population of biofilm cells was reduced by more than 67%. Additionally, AEW triggered EPS disruption, as indicated by the deformation of the carbohydrate C-O-C bond and deformation of the aromatic rings in the amino acids tyrosine and phenylalanine. These deformations were identified by EPS chemical analysis and Raman spectroscopic analysis. Scanning electron microscopy (SEM) images confirmed that the breakup and detachment of biofilm were enhanced after AEW treatment. Further, AEW also eradicated biofilms formed by both Gram-negative bacteria (Vibrio parahaemolyticus) and Gram-positive bacteria (Listeria monocytogenes) and was observed to inactivate the detached cells which are a potential source of secondary pollution. This study demonstrates that AEW could be a reliable foodborne pathogen biofilm disrupter and an eco-friendly alternative to sanitizers traditionally used in the food industry.