Direct and noninvasive assessment of parafoveal capillary leukocyte velocity
Direct and noninvasive assessment of parafoveal capillary leukocyte velocity
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DOI：
10.1016/j.ophtha.2005.06.033
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发表时间：
2005-12-01
期刊：
影响因子：
13.7
通讯作者：
Roorda, A
中科院分区：
文献类型：
--
作者：
Martin, JA;Roorda, A
目的：白细胞速度的改变与许多视网膜疾病过程有关。然而，视网膜毛细血管中白细胞速度的直接和客观的评估受到了依赖侵入性对比染料的限制，该染料仅允许白细胞在短时间内可视化。自适应光学最近在扫描激光检眼镜（AOSLO）的应用，使长期成像旁白细胞运动可能没有对比染料。在这项研究中，使用AOSLO，我们展示了一种新的方法来调查视网膜旁毛细血管白细胞速度。设计：实验研究。参与者：6名年龄在25至35岁之间的正常健康受试者，具有清晰的眼介质。方法：使用AOSLO对所有受试者的视网膜旁区进行成像。主要结果测量：白细胞速度测定在旁毛细血管，包括中心凹无血管区边界。结果：6例受试者的白细胞速度平均为1.37 mm/s，范围为0.77 ~ 2.10 mm/s。白细胞是不可见的，在所有parafterialcapillars.Conclusions：parafterialcapillars. Conclusions白细胞的速度可以直接和非侵入性测量，而不使用造影剂使用AOSLO。
Purpose: Alterations in leukocyte velocity have been implicated in many retinal disease processes. However, direct and objective assessment of leukocyte velocity in retinal capillaries has been limited by a reliance on invasive contrast dyes that allow leukocyte visualization only for a short time span. The recent application of adaptive optics in a scanning laser ophthalmoscope (AOSLO) has made long-term imaging of parafoveal leukocyte movement possible without contrast dyes. In this study, using the AOSLO, we demonstrate a new method to investigate retinal parafoveal capillary leukocyte velocity.Design: Experimental study.Participants: Six normal healthy subjects ranging from 25 to 35 years of age with clear ocular media.Methods: The parafoveal zone of the retina was imaged in all subjects using an AOSLO.Main Outcome Measures: Leukocyte velocity was determined in the parafoveal capillaries including the foveal avascular zone border. Leukocyte velocity was measured directly from movie segments in which the leukocytes were clearly visible.Results: The mean parafoveal leukocyte velocity for 6 subjects was 1.37 mm/second, ranging from 0.77 to 2.10 mm/second. Leukocytes were not visible in all parafoveal capillaries.Conclusions: Parafoveal capillary leukocyte velocity can be directly and noninvasively measured without the use of contrast dyes using an AOSLO.