Effect of subinhibitory concentrations of antibiotics on the adhesion of Streptococcus pyogenes to pharyngeal epithelial cells
Effect of subinhibitory concentrations of antibiotics on the adhesion of Streptococcus pyogenes to pharyngeal epithelial cells
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亚抑菌浓度抗生素对化脓性链球菌粘附咽上皮细胞的影响
DOI：
10.1128/aac.20.5.563
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发表时间：
1981
影响因子：
4.9
通讯作者：
T. Wadström
中科院分区：
文献类型：
--
作者：
S. Tylewska;S. Hjertén;T. Wadström
用疏水作用层析和自由区带电泳法研究了M蛋白阳性(M+)和M蛋白阴性(M-)的12型化脓性链球菌的细胞表面疏水性和电荷性。与M-菌株相比，M+菌株具有更强的疏水性和更强的负电荷表面。当M+菌株在不同抗生素的亚最低抑菌浓度下培养时，其疏水性要么降低，要么不变。与M+相比，M+菌对咽上皮细胞的粘附性更强；然而，在青霉素和利福平的抑制浓度低于最低浓度的情况下，这两种菌株的粘附率都有所下降。四环素只降低M+菌的粘附性，而头孢菌素和多粘菌素(耐药株)不影响M+菌的粘附性或疏水性。在利福平和青霉素作用下，M+细菌表面负电荷显著增加，而M-细菌的表面负电荷几乎没有变化。讨论了这些变化对抑制感染的贡献。
The hydrophobicity and charge of the cell surface of M protein-positive (M+) and the less virulent M protein-negative (M-) strains of type 12 Streptococcus pyogenes have been studied, respectively, by hydrophobic interaction chromatography and free zone electrophoresis. The M+ strain had a more hydrophobic and a more negatively charged surface than the M- strain. When the M+ strain was cultivated in the presence of sub-minimum inhibitory concentrations of different antibiotics, its hydrophobicity either decreased or did not change. The M+ organisms adhered to pharyngeal epithelial cells more avidly than M+; however, cultivation of both strains with sub-minimum inhibitory concentrations of penicillin and rifampin led to the decrease in adhesion. Tetracycline caused a decrease in adhesion for the M+ strain only, whereas cephalothin and polymyxin (to which the strains are resistant) did not affect adhesion or hydrophobicity of the M+ organisms. The negative surface charge of the M+ bacteria increased considerably upon exposure to rifampin and penicillin, and the M- bacteria exhibited small or no change. The contributions of these changes to suppression of infections are discussed.