Introduction to Atomic Force Microscopy
Introduction to Atomic Force Microscopy
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DOI：
10.1007/978-1-61779-282-3_11
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
de Pablo, Pedro J.
中科院分区：
文献类型：
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作者：
de Pablo, Pedro J.
原子力显微镜（AFM）是一种非常宝贵的工具，不仅可以获得高分辨率的地形图像，而且可以确定样品的某些物理性质，如机械性能和成分。除了广泛的应用，从材料科学到生物学，这项技术可以在许多环境中操作，只要试样附着在表面，包括环境空气，超高真空（UHV），最重要的是生物学，在液体中。该技术的多功能性也反映在可以处理的样品的大小范围广泛，例如原子，分子，分子聚集体和细胞。事实上，这项技术使生物学问题能够从单分子的角度来解决，它不仅允许看到而且还允许触摸所研究的材料（即纳米尺度的机械操作），这是表征其特征的基本信息来源。特别是，纳米尺度生物分子聚集体力学性能的研究为构建单粒子生物机器的力学化学结构/功能模型提供了重要的数据来源，扩展和补充了体体实验获得的信息。
Atomic force microscopy (AFM) is an invaluable tool not only to obtain high-resolution topographical images, but also to determine certain physical properties of specimens, such as their mechanical properties and composition. In addition to the wide range of applications, from materials science to biology, this technique can be operated in a number of environments as long as the specimen is attached to a surface, including ambient air, ultra high vacuum (UHV), and most importantly for biology, in liquids. The versatility of this technique is also reflected by the wide range of sizes of the sample that can dealt with, such as atoms, molecules, molecular aggregates, and cells. Indeed, this technique enables biological problems to be tackled from the single-molecule point of view and it allows not only to see but also to touch the material under study (i.e., mechanical manipulation at the nanoscale), a fundamental source of information for its characterization. In particular, the study of the mechanical properties at the nanoscale of biomolecular aggregates constitute an important source of data to elaborate mechano-chemical structure/function models of single-particle biomachines, expanding and complementing the information obtained from bulk experiments.