Distribution of vanilloid receptors in the rat laryngeal innervation
Distribution of vanilloid receptors in the rat laryngeal innervation
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DOI：
10.1080/00016480310000674
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发表时间：
2004-05-01
影响因子：
1.4
通讯作者：
Hisa, Y
中科院分区：
文献类型：
--
作者：
Koike, S;Uno, T;Hisa, Y
目的：辣椒素通过香草样受体选择性激活伤害感觉神经元。本研究探讨了香草样受体亚型1 （VR1）和香草样受体样蛋白1 （VRL-1）在大鼠喉部的分布。材料与方法：采用免疫组化方法测定VR1和VRL-1的分布。采用免疫组织化学双标记技术研究了香草素受体与胆碱乙酰转移酶（cChAT）、血管活性肠多肽（VIP）、P物质（SP）和神经元一氧化氮合酶（nNOS）的共定位。结果：喉上皮和喉固有层均检测到vrl -1阳性纤维。vr1阳性的神经纤维见于固有层，但未见于粘膜上皮。VR1-和vrl -1阳性细胞分布于喉内神经节，可见辣椒素受体与VIP、nNOS和cChAT共定位。结论：这些辣椒素受体参与了大鼠喉部的副交感神经支配和伤害感觉。
Objective-Capsaicin is known to selectively activate nociceptic sensory neurons through vanilloid receptors. In this study we investigated the distribution of vanilloid receptor subtype 1 (VR1) and vanilloid receptor-like protein 1 (VRL-1) in the rat larynx.Material and Methods-The distributions of VR1 and VRL-1 were determined immunohistochemically. The colocalization of vanilloid receptors with common choline acetyltransferase (cChAT), vasoactive intestinal polypeptide (VIP), substance P (SP) and neuronal nitric oxide synthase (nNOS) was also studied using an immunohistochemical double-labeling technique.Results-VRL-1-positive fibers were detected in the laryngeal epithelium and lamina propria. VR1-positive nerve fibers were seen in the lamina propria but not in the mucosal epithelium. VR1- and VRL-1-positive cells were distributed in the intralaryngeal ganglia and colocalization of capsaicin receptors with VIP, nNOS and cChAT was seen.Conclusion-These findings suggest that these capsaicin receptors participate in the parasympathetic innervation as well as in nociception of the rat larynx.