The SAM-responsive S(MK) box is a reversible riboswitch.
The SAM-responsive S(MK) box is a reversible riboswitch.
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SAM响应S（MK）框是可逆的核糖开关。
DOI：
10.1111/j.1365-2958.2010.07410.x
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发表时间：
2010-12
影响因子：
3.6
通讯作者：
Henkin TM
中科院分区：
文献类型：
--
作者：
Smith AM;Fuchs RT;Grundy FJ;Henkin TM
The SMK (SAM-III) box is an S-adenosylmethionine (SAM)-responsive riboswitch found in the 5′ untranslated region of metK genes, encoding SAM synthetase, in many members of the Lactobacillales. SAM binding causes a structural rearrangement in the RNA that sequesters the Shine-Dalgarno (SD) sequence by pairing with a complementary anti-SD (ASD) sequence; sequestration of the SD sequence inhibits binding of the 30S ribosomal subunit and prevents translation initiation. We observed a slight increase in the half-life of the metK transcript in vivo when Enterococcus faecalis cells were depleted for SAM, but no significant change in overall transcript abundance, consistent with the model that this riboswitch regulates at the level of translation initiation. The half-life of the SAM-SMK box RNA complex in vitro is shorter than that of the metK transcript in vivo, raising the possibility of reversible binding of SAM. We used a fluorescence assay to directly visualize reversible switching between the SAM-free and SAM-bound conformations. We propose that the SMK box riboswitch can make multiple SAM-dependent regulatory decisions during the lifetime of the transcript in vivo, acting as a reversible switch that allows the cell to respond rapidly to fluctuations in SAM pools by modulating expression of the SAM synthetase gene.
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DOI：
10.1073/pnas.85.4.1033
发表时间：
1988-02-01
影响因子：
11.1
作者：
SAMPSON, JR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
64.5
作者：
GRUNDY, FJ;HENKIN, TM
通讯作者：
HENKIN, TM
影响因子：
3.2
作者：
McDaniel, Brooke A.;Grundy, Frank J.;Henkin, Tina M.
通讯作者：
Henkin, Tina M.
DOI：
10.1038/nsb967
发表时间：
2003-09-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Winkler, WC;Nahvi, A;Breaker, RR
通讯作者：
Breaker, RR
DOI：
10.1073/pnas.0630422100
发表时间：
2003-03-18
影响因子：
11.1
作者：
McDaniel, BAM;Grundy, FJ;Henkin, TM
通讯作者：
Henkin, TM