Interference with brachyury function inhibits convergent extension, causes apoptosis, and reveals separate requirements in the FGF and activin signalling pathways
Interference with brachyury function inhibits convergent extension, causes apoptosis, and reveals separate requirements in the FGF and activin signalling pathways
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DOI：
10.1006/dbio.1999.9330
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发表时间：
1999-09-01
影响因子：
2.7
通讯作者：
Smith, JC
中科院分区：
文献类型：
--
作者：
Conlon, FL;Smith, JC
Brachyury在脊椎动物发育过程中的中胚层形成中起着关键作用。该基因的缺失导致后中胚层的缺失和脊索分化失败，而短尾畸形在爪蟾的预期外胚层中的错误表达导致异位中胚层形成。因此，短尾畸形对于后中胚层的形成是必要的和充分的。在这里，我们提出了一个详细的细胞和分子分析的后果，抑制Brachyury功能在非洲爪蟾的发展。我们的研究结果表明，Brachyury是必需的原肠胚的收敛延伸运动，中胚层分化响应FGF，和后中胚层细胞在非洲爪蟾和小鼠的生存。(C)北京：科学出版社.
Brachyury plays a key role in mesoderm formation during vertebrate development. Absence of the gene results in loss of posterior mesoderm and failure of the notochord to differentiate, while misexpression of Brachyury in the prospective ectoderm of Xenopus results in ectopic mesoderm formation. Brachyury is therefore both necessary and sufficient for posterior mesoderm formation. Here we present a detailed cellular and molecular analysis of the consequences of inhibiting Brachyury function during Xenopus development. Our results show that Brachyury is required for the convergent extension movements of gastrulation, for mesoderm differentiation in response to FGF, and for the survival of posterior mesodermal cells in both Xenopus and mouse. (C) 1999 Academic Press.