CENTRAL-NERVOUS-SYSTEM NEURONS MIGRATE ON ASTROGLIAL FIBERS FROM HETEROTYPIC BRAIN-REGIONS INVITRO
CENTRAL-NERVOUS-SYSTEM NEURONS MIGRATE ON ASTROGLIAL FIBERS FROM HETEROTYPIC BRAIN-REGIONS INVITRO
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DOI：
10.1073/pnas.87.12.4543
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发表时间：
1990-06-01
影响因子：
11.1
通讯作者：
HATTEN, ME
中科院分区：
文献类型：
--
作者：
GASSER, UE;HATTEN, ME
在发育中的哺乳动物大脑的不同区域，神经元遵循放射状胶质细胞的过程，以非常不同的轨迹到达特定神经元层中的目的地。为了研究神经元是否沿着沿着胶质细胞在一个给定的区域或胶质细胞是否提供了一个允许的基板迁移在不同的大脑区域，我们纯化的神经元和星形胶质细胞从发展中的小脑和海马和分析异型胶质纤维的神经元迁移与时间推移，视频增强差分干涉显微镜在体外。从出生后早期大鼠小脑纯化的颗粒神经元迁移到从胚胎晚期或出生后早期大鼠海马纯化的胶质细胞的星形胶质细胞突起上，其细胞学、神经元-胶质细胞关系和运动动力学与在体外迁移到小脑星形胶质细胞突起上的小鼠颗粒细胞无法区分[Edmondson，J. C.和哈滕，M. E.等人（1987）J. Neurosci. 7，1928-1934]。在相互组合中，海马神经元在小脑神经胶质突起上的迁移方式也与沿沿着同型海马神经胶质纤维的迁移非常相似[Gasser，U. E.和哈滕，M. E.（1990）J. Neurosci，10，1276-1285]。在所有的情况下，迁移神经元有一个特征性的外观，并列其细胞索马对胶质纤维和迁移方向延伸的一个能动的，领先的过程，包围胶质纤维与短丝状伪足和板状伪足。通过视频显微镜观察，神经元通过索马移位沿沿着同型和异型胶质突起移动，并且没有被引导突起“纯化”。当神经元移动时，细胞核仍留在细胞的后部，胞质小泡从索马向前移动进入前导突。神经元沿着异型胶质基质的运动动力学，包括运动的速度和周期性，与神经元沿沿着同型胶质基质迁移的动力学相同。这些实验表明，神经元沿沿着胶质纤维运动的机制在发育过程中在这两个脑区是保守的。
In different regions of the developing mammalian brain, neurons follow the processes of radial glial cells over very different trajectories to reach their destinations in specific neuronal layers. To investigate whether the movement of neurons along glia in a given region or whether glia provide a permissive substrate for migration in different brain regions, we purified neurons and astroglial cells from developing cerebellum and hippocampus and analyzed neuronal migration on heterotypic glial fibers with time-lapse, video-enhanced differential interference microscopy in vitro. Granule neurons purified from early postnatal rat cerebellum migrated on astroglial processes of glia purified from late embryonic or early postnatal rat hippocampus with a cytology, neuron-glial relationship, and dynamics of movement that were indistinguishable from those of mouse granule cells migrating on cerebellar astroglial processes in vitro [Edmondson, J. C. and Hatten, M. E. (1987) J. Neurosci. 7, 1928-1934]. In the reciprocal combination, hippocampal neurons migrated on cerebellar glial processes in a manner that was also remarkably similar to mgiration along homotypic, hippocampal glial fibers [Gasser, U. E. and Hatten, M. E. (1990) J. Neurosci, 10, 1276-1285]. In all cases, migrating neurons had a characteristic appearance, apposing their cell soma against the glial fiber and extending in the direction of migration a motile, leading process that enfolded the glial fiber with short filopodia and lamellipodia. As seen by video microscopy, neurons moved along homotypic and heterotypic glial processes by translocation of the soma and were not "purified" forward by the leading process. As the neuron moved, the nucleus remained in the posterior portion of the cell and cytoplasmic vesicles moved forward from the soma into the leading process. The dynamics of the movement of neurons along heterotypic glial substrates, including the speed and periodicity of motion, was identical to that of neurons migrating along homotypic glial substrates. These experiments suggest that the mechanism of movement of neurons along glial fibers is conserved in these two brain regions during development.