Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates
Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates
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DOI：
10.1016/j.biomaterials.2010.04.019
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发表时间：
2010-08-01
期刊：
影响因子：
14
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Lu, Hongxu;Ko, Young-Gwang;Chen, Guoping
胶原三维多孔支架已广泛应用于组织工程和再生医学领域。在这项研究中，我们制作了漏斗状胶原海绵与开放的表面孔结构的冷冻干燥方法，使用压花冰颗粒作为模板。通过控制冰粒的大小和冷冻温度，制备了具有不同孔结构的胶原海绵。为探讨不同孔隙结构对软骨再生的影响，采用漏斗状胶原海绵培养牛关节软骨细胞。用400 μ π ι冰颗粒模板和-3 ° C的冷冻温度制备的支架导致最佳的细胞分布、ECM产生和软骨形成。尽管用400 μ m冰颗粒模板和1 ℃冷冻温度以及720 μ m冰颗粒和-3 ℃冷冻温度制备的漏斗状胶原海绵显示出均匀的细胞分布，但细胞接种效率和每个细胞的sGAG量低。然而，用400 μ m冰颗粒模板和-5 ℃或-10 ℃的冷冻温度制备的支架对细胞分布和软骨形成的改善效果有限。没有冰颗粒的对照胶原海绵不能支持均匀软骨样组织的形成。这些结果表明，漏斗状胶原海绵是上级的控制胶原海绵和400 μ m的冰颗粒模板在-3 ℃制备的支架是软骨组织工程的最佳。(C)2010爱思唯尔有限公司版权所有。
Three-dimensional porous scaffolds of collagen have been widely used for tissue engineering and regenerative medicine. In this study, we fabricated funnel-like collagen sponges with open surface pore structures by a freeze-drying method that used embossing ice particulates as a template. By controlling the size of the ice particulates and the temperature of freezing, collagen sponges with different pore structures were prepared. To investigate the effects of different pore structures on cartilage regeneration, the funnel-like collagen sponges were used to culture bovine articular chondrocytes. Scaffolds that were prepared with 400 pm ice particulate templates and a freezing temperature of -3 degrees C resulted in the best cell distribution, ECM production, and chondrogenesis. Although funnel-like collagen sponges prepared with 400 mu m ice particulate templates and a freezing temperature of 1 degrees C and 720 mu m ice particulates and a freezing temperature of -3 degrees C, showed even cell distribution, the cell seeding efficiencies and sGAG amount per cell were low. However, the scaffolds prepared with 400 mu m ice particulate templates and a freezing temperature of -5 degrees C or -10 degrees C showed a limited effect on the improvement of cell distribution and chondrogenesis. Control collagen sponges without ice particulates failed to support the formation of homogenous cartilage-like tissue. These results indicate that funnel-like collagen sponges were superior to control collagen sponges and that scaffolds prepared with 400 mu m ice particulate templates at -3 degrees C were optimal for cartilage tissue engineering. (C) 2010 Elsevier Ltd. All rights reserved.