Molecular Evolution of Calmodulin-Like Domain Protein Kinases (CDPKs) in Plants and Protists
Molecular Evolution of Calmodulin-Like Domain Protein Kinases (CDPKs) in Plants and Protists
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DOI：
10.1007/s002390010211
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发表时间：
2001-09
影响因子：
3.9
通讯作者：
Xiaorong S. Zhang;Jung H. Choi
中科院分区：
文献类型：
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作者：
Xiaorong S. Zhang;Jung H. Choi
钙调素样结构域蛋白激酶（CDPKs）基因在植物和泡状原生生物中已被发现。为了研究CDPK基因家族的分子进化，我们对CDPK基因组序列进行了系统发育分析。内含子的分析支持系统发育分析;具有相似内含子/外显子结构的CDPK基因在系统发育树上被分组在一起。保守的内含子支持植物CDPK、CDPK相关激酶和磷酸烯醇式丙酮酸羧化酶激酶的单系起源。植物CDPKs分为两个主要分支。同一分支上的植物CDPK基因与原生生物CDPK基因具有共同的内含子位置。原生生物和植物CDPKs共有的内含子可能起源于植物从泡孔动物分化之前。此外，原生生物CDPKs的钙调素样结构域具有与动物和真菌钙调素基因相同的内含子位置。这些结果，连同一个高度保守的零相内含子的存在下，正好位于钙调素样结构域的开始，表明祖先CDPK基因可能起源于蛋白激酶和钙调素基因的融合促进重组古老的内含子。
Many genes for calmodulin-like domain protein kinases (CDPKs) have been identified in plants and Alveolate protists. To study the molecular evolution of the CDPK gene family, we performed a phylogenetic analysis of CDPK genomic sequences. Analysis of introns supports the phylogenetic analysis; CDPK genes with similar intron/exon structure are grouped together on the phylogenetic tree. Conserved introns support a monophyletic origin for plant CDPKs, CDPK-related kinases, and phosphoenolpyruvate carboxylase kinases. Plant CDPKs divide into two major branches. Plant CDPK genes on one branch share common intron positions with protist CDPK genes. The introns shared between protist and plant CDPKs presumably originated before the divergence of plants from Alveolates. Additionally, the calmodulin-like domains of protist CDPKs have intron positions in common with animal and fungal calmodulin genes. These results, together with the presence of a highly conserved phase zero intron located precisely at the beginning of the calmodulin-like domain, suggest that the ancestral CDPK gene could have originated from the fusion of protein kinase and calmodulin genes facilitated by recombination of ancient introns.