Deformation response of red blood cells in oscillatory shear flow.
Deformation response of red blood cells in oscillatory shear flow.
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红细胞在振荡剪切流中的变形反应。
DOI：
10.1152/ajpheart.1990.259.4.h1071
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
T. Shiga
中科院分区：
文献类型：
--
作者：
T. Nakajima;K. Kon;N. Maeda;K. Tsunekawa;T. Shiga
研究了红细胞的变形特性，着重研究了红细胞在振荡剪切力作用下的变形响应。将红细胞分成不同密度的亚群，即，低密度和高密度细胞。分级细胞的变形响应进行了评价，用流变仪改变其细胞内粘度和振荡频率的所施加的剪切应力，和变形响应的决定因素进行了比较与那些固定的剪切应力下的整个细胞变形。当分离的细胞暴露于正弦振荡剪切应力时，细胞发生振荡变形。低密度单元的变形程度对应于高达2.7Hz的振荡频率的所施加的剪切应力的大小。同时，对于高密度细胞，在高达1.7 Hz的频率下观察到与施加的剪应力相对应的振荡变形。结果表明，细胞内粘度是决定红细胞对振荡剪切力变形反应的重要因素。
The characteristics of red cell deformation were studied, focusing on deformation response of the cells subjected to oscillatory shear stress. Red blood cells were fractionated into subpopulations of different densities, i.e., low-density and high-density cells. The deformation response of the fractionated cells was evaluated with a rheoscope varying their intracellular viscosity and oscillation frequency of the applied shear stress, and determinants of the deformation response were compared with those of whole cell deformation under stationary shear stress. When the fractionated cells were exposed to sinusoidally oscillated shear stress, the cells underwent oscillatory deformation. The degree of deformation of the low-density cells correspond to the magnitude of the applied shear stress up to an oscillation frequency of 2.7 Hz. Meanwhile, such an oscillatory deformation as to correspond to the applied shear stress was observed up to 1.7 Hz for the high-density cells. It was suggested that intracellular viscosity was an important factor to determine the deformation response of red cells to oscillatory shear stress.