Relationship between intercellular permeability and junction organization in the preimplantation mouse embryo.
Relationship between intercellular permeability and junction organization in the preimplantation mouse embryo.
复制标题
植入前小鼠胚胎细胞间通透性与连接组织之间的关系。
DOI：
10.1016/0012-1606(78)90310-x
复制
发表时间：
1978
影响因子：
2.7
通讯作者：
C. Stackpole
中科院分区：
文献类型：
--
作者：
T. Magnuson;J. B. Jacobson;C. Stackpole
检查植入前小鼠胚胎对不同分子量分子的细胞间渗透性。使用免疫手术后免疫荧光，没有8细胞胚胎，约10%的早期桑椹胚，50%的晚期桑椹胚，和90%的早期囊胚被发现阻止非特异性抗小鼠胸腺细胞血清扩散到细胞间隙。辣根过氧化物酶和微过氧化物酶扩散到细胞间隙的可行的胚胎也阻碍了一些桑椹胚和所有早期囊胚保持在冰上。在37°C下检查的一些早期囊胚的囊胚腔中发现的过氧化物酶示踪剂似乎是胞饮作用和跨细胞运动的结果。镧进入戊二醛固定的胚胎的细胞间扩散仅受到早期囊胚的阻碍。这些结果表明，在囊胚腔形成之前，在早期小鼠胚胎的外部细胞之间建立了渗透性密封。此外，冷冻断裂电子显微镜揭示了闭锁小带连接组织的相关变化，表明细胞间渗透性屏障的形成和随后的囊胚腔形成可能取决于这些连接组装的完成。
Preimplantation mouse embryos were examined for intercellular permeability to molecules of different molecular weights. Using immunosurgery followed by immunofluorescence, none of the eight-cell embryos, approximately 10% of the early morulae, 50% of the late morulae, and 90% of the early blastocysts were found to block nonspecific anti-mouse thymocyte serum from diffusing into intercellular spaces. Diffusion of horseradish peroxidase and microperoxidase into intercellular spaces of viable embryos was also impeded by some morulae and by all early blastocysts maintained on ice. Peroxidase tracers found within the blastocoel cavity of some early blastocysts examined at 37°C appear to be a result of pinocytosis and transcellular movement. Intercellular diffusion of lanthanum into glutaraldehyde-fixed embryos was impeded only by early blastocysts. These results suggest that a permeability seal is established between external cells of the early mouse embryo prior to blastocoel formation. In addition, freeze-fracture electron microscopy revealed a correlated change in zonula occludens junction organization, indicating that formation of an intercellular permeability barrier and subsequent blastocoel formation may depend upon completion of assembly of these junctions.