Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells
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DOI：
10.1186/1756-9966-32-11
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发表时间：
2013-03-02
影响因子：
11.3
通讯作者：
Yu, ZhiJuan
中科院分区：
文献类型：
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作者：
Li, Shu;Ma, XiaoXin;Yu, ZhiJuan
目的：探讨HER-2/neu介导的考克斯-2/P450 arom信号在雌激素依赖性子宫内膜癌中的作用。方法：构建重组真核表达载体pcDNA3.1-HER-2/neu，并转染石川子宫内膜癌细胞。实时荧光定量PCR和Western blotting检测转染细胞中考克斯-2和P450 arom的表达。结果：转染后的细胞中HER-2/neu表达量明显高于转染前。结论：HER-2/neu通过激活考克斯-2/P450 arom信号，促进子宫内膜癌细胞雌激素的自分泌。
Objectives: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma.Methods: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA.Results: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells.Conclusion: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal.