PATHWAYS OF 4-HYDROXYBENZOATE DEGRADATION AMONG SPECIES OF BACILLUS
PATHWAYS OF 4-HYDROXYBENZOATE DEGRADATION AMONG SPECIES OF BACILLUS
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DOI：
10.1128/jb.127.1.204-210.1976
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发表时间：
1976-01-01
影响因子：
3.2
通讯作者：
CRAWFORD, RL
中科院分区：
文献类型：
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作者：
CRAWFORD, RL
描述了3株芽孢杆菌降解对羟基苯甲酸酯的途径。当B. brevis菌株PHB-2在4-羟基苯甲酸盐上生长，所述4-羟基苯甲酸盐是β-葡聚糖的原儿茶酸分支的酶。酮己二酸途径被诱导。B。在4-羟基苯甲酸盐上生长后，环状菌菌株3含有高水平的原儿茶酸2，3-双加氧酶途径的酶。B。侧孢菌菌株PHB-7a通过新的反应顺序降解4-羟基苯甲酸酯。在4-羟基苯甲酸盐上生长后，菌株PHB-7a含有高水平的龙胆酸加氧酶（EC 1.13.11.4）和马来酸丙酮酸水解酶。菌株PHB-7a的全细胞（在4-羟基苯甲酸上生长）当在1 mM α，β-羟基苯甲酸存在下孵育时从4-羟基苯甲酸积累2，5-二羟基苯甲酸（龙胆酸盐）。α的- 联吡啶。菌株PHB-7a似乎将4-羟基苯甲酸盐转化为龙胆酸盐，龙胆酸盐通过谷胱甘肽非依赖性龙胆酸途径进一步降解。这些途径描绘提供了芽孢杆菌属的证据。都来自不同的进化背景
The pathways used by 3 bacterial strains of the genus Bacillus to degrade 4-hydroxybenzoate are delineated. When B. brevis strain PHB-2 is grown on 4-hydroxybenzoate, enzymes of the protocatechuate branch of the .beta.-ketoadipate pathway are induced. B. circulans strain 3 contains high levels of the enzymes of the protocatechuate 2,3-dioxygenase pathway after growth on 4-hydroxybenzoate. B. laterosporus strain PHB-7a degrades 4-hydroxybenzoate by a novel reaction sequence. After growth on 4- hydroxybenzoate, strain PHB-7a contains high levels of gentisate oxygenase (EC 1.13.11.4) and maleypyruvate hydrolase. Whole cells of strain PHB-7a (grown on 4-hydroxybenzoate) accumulate 2,5-dihydroxybenzoate (gentisate) from 4-hydroxybenzoate when incubated in the presence of 1 mM .alpha.,.alpha.''-dipyridyl. Strain PHB-7a appears to convert 4-hydroxybenzoate to gentisate, which is further degraded by the glutathione-independent gentistic acid pathway. These pathway delineations provide evidence that Bacillus spp. are derived from a diverse evolutionary background.