A contextual framework for characterizing motility and chemotaxis mutants in Dictyostelium discoideum
A contextual framework for characterizing motility and chemotaxis mutants in Dictyostelium discoideum
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DOI：
10.1023/a:1024459124427
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发表时间：
2002-10-01
影响因子：
2.7
通讯作者：
Zhang, H
中科院分区：
文献类型：
--
作者：
Soll, DR;Wessels, D;Zhang, H
在自然聚集过程中，变形网骨藻通过细胞群向外传递cAMP信号，作为对称的非耗散波。每个细胞又以特定的方式响应波的不同相位。在每个波的前部，每个细胞经历cAMP的增加的时间梯度和正的空间梯度;在每个波的峰值处，每个细胞经历抑制运动的cAMP浓度;并且在每个波的后部，每个细胞经历cAMP的减少的时间梯度和负的空间梯度。协议被描述为在没有趋化信号的情况下分析突变细胞的基本运动行为，并测试突变细胞对自然波的个体时间、空间和浓度分量的响应。这样的分析结果，然后可以用来开发现实的模型细胞运动和趋化性。实例中描述了这种上下文框架已被应用于突变细胞系。这些突变体研究的结果导致了一个模型，其中来自波的不同相位的独立平行调节通路影响不同的相位特异性行为。
In the natural aggregation process, Dictyostelium amoebae relay the cAMP signal outwardly through the cell population as symmetric, nondissipating waves. Each cell in turn responds in a specific manner to the different phases of the wave. In the front of each wave, each cell experiences an increasing temporal gradient and positive spatial gradient of cAMP; at the peak of each wave, each cell experiences a cAMP concentration inhibitory to locomotion; and in the back of each wave, each cell experiences a decreasing temporal and negative spatial gradient of cAMP. Protocols are described to analyze the basic motile behavior of mutant cells in the absence of a chemotactic signal, and to test the responsiveness of mutant cells to the individual temporal, spatial and concentration components of a natural wave. The results of such an analysis can then be used to develop realistic models of cell motility and chemotaxis. Examples are described in which this contextual framework has been applied to mutant cell lines. The results of these mutant studies result in a model in which independent parallel regulatory pathways emanating from different phases of the wave effect different phase-specific behaviors.