Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development
Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development
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DOI：
10.1002/1873-3468.14570
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发表时间：
2023-01-24
期刊：
影响因子：
3.5
通讯作者：
Ohme-Takagi, Masaru
中科院分区：
文献类型：
--
作者：
Zhang, Yilin;Maruyama, Daisuke;Ohme-Takagi, Masaru
开花植物种子发育过程中胚乳-胚的发育受信号交换的协调调控。然而，这种相互控制机制尚未明确确定。在这项研究中，我们确定了胚乳特异性基因，LBD 35，在胚胎发育依赖的方式表达，通过比较转录组和细胞学分析的双受精和单受精种子制备使用的kokopelli突变体，它经常诱导单受精事件。使用LBD 35作为中央细胞受精事件的标记物的转录组分析确定了141个基因，包括31个富含半胱氨酸的小肽的基因，以双受精依赖的方式表达。这些结果揭示了胚乳发育过程中可能存在的调控胚乳基因表达的胚胎信号，为鉴定胚乳-胚发育过程中参与胚乳-胚间通讯的基因提供了一种可行的方法。
Endosperm-embryo development in flowering plants is regulated coordinately by signal exchange during seed development. However, such a reciprocal control mechanism has not been clearly identified. In this study, we identified an endosperm-specific gene, LBD35, expressed in an embryonic development-dependent manner, by a comparative transcriptome and cytological analyses of double-fertilized and single-fertilized seeds prepared by using the kokopelli mutant, which frequently induces single fertilization events. Transcriptome analysis using LBD35 as a marker of the central cell fertilization event identified that 141 genes, including 31 genes for small cysteine-rich peptides, are expressed in a double fertilization-dependent manner. Our results reveal possible embryonic signals that regulate endosperm gene expression and provide a practicable method to identify genes involved in the communication during endosperm-embryo development.