Strategies for single particle manipulation using acoustic and flow fields
Strategies for single particle manipulation using acoustic and flow fields
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DOI：
10.1016/j.ultras.2009.09.004
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发表时间：
2010-02-01
期刊：
影响因子：
4.2
通讯作者：
Gutmann, S.
中科院分区：
文献类型：
--
作者：
Oberti, S.;Moeller, D.;Gutmann, S.
声辐射力经常被用于操纵大量微米级的悬浮颗粒。声驻波场的性质是，它们存在于整个流体体积中；这意味着它们非常适合这样的操作，所有悬浮颗粒在暴露时同时发生反应。这里，利用这种同时定位能力来沿通道中心线预先对准颗粒，从而可以通过外部工具连续移除颗粒以进行进一步分析。这允许在单个粒子操纵过程中实现一定程度的自动化，因为粒子位置的初始识别不再是必需的，而是预先确定的。已经确定了两个应用领域的研究。首先，介绍了使用微夹持器操纵共聚物珠和细胞的方法。然后，将介绍通过使用声学操纵和层流将晶体定位到环路中来为结晶学分析准备样品。(C)2009爱思唯尔B.V.保留所有权利。
Acoustic radiation forces have often been used for the manipulation of large amounts of micrometer sized suspended particles. The nature of acoustic standing wave fields is such that they are present throughout the whole fluidic volume; this means they are well suited to such operations, with all suspended particles reacting at the same time upon exposure. Here, this simultaneous positioning capability is exploited to pre-align particles along the centerline of channels, so that they can successively be removed by means of an external tool for further analysis. This permits a certain degree of automation in single particle manipulation processes to be achieved as initial identification of particles' location is no longer necessary, rather predetermined. Two research fields in which applications are found have been identified. First, the manipulation of copolymer beads and cells using a microgripper is presented. Then, sample preparation for crystallographic analysis by positioning crystals into a loop using acoustic manipulation and a laminar flow will be presented. (C) 2009 Elsevier B.V. All rights reserved.