USE OF DIPOSABLE MICRO TISSUE CULTURE PLATES FOR ANTIVIRAL AND INTERFERON INDUCTION STUDIES
USE OF DIPOSABLE MICRO TISSUE CULTURE PLATES FOR ANTIVIRAL AND INTERFERON INDUCTION STUDIES
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DOI：
10.1128/aem.22.5.797-801.1971
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
HUFFMAN, JH
中科院分区：
文献类型：
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作者：
SIDWELL, RW;HUFFMAN, JH
开发了一种重复性测试系统，需要少量的测试化合物来评估抗病毒和干扰素诱导活性。在抗病毒实验中，KB细胞生长在一次性聚苯乙烯微孔板上，上面覆盖一层标准的国产保温膜。系统中使用的病毒有1型和2型单纯疱疹病毒、牛痘病毒、3型腺病毒、黏液瘤病毒、假狂犬病毒、3型副流感病毒、1A型和13型鼻病毒、水泡性口炎病毒、柯萨奇病毒B和2型脊髓灰质炎病毒。抑制病毒的细胞致病作用是评价抗病毒活性的主要标准。降低细胞和上清液病毒滴度作为第二评价手段。微孔板系统适用于测定对病毒的预防、治疗和灭活效果。采用小鼠L-929细胞进行干扰素诱导研究，以水疱性口炎病毒作为干扰素活性指标。在该微孔板系统中评估的已知活性化合物的活性与在体外系统中观察到的活性相似。
A reproducible test system requiring small amounts of test compound was developed for evaluating antiviral and interferon-inducing activity. In the antiviral experiments, KB cells were grown in disposable polystyrene microplates covered with a standard domestic plastic wrap. Viruses used in the system were types 1 and 2 herpes simplex virus, vaccinia virus, type 3 adenovirus, myxoma virus, pseudorabies virus, type 3 parainfluenza virus, types 1A and 13 rhinovirus, vesicular stomatitis virus, coxsackievirus B, and type 2 poliovirus. Inhibition of viral cytopathogenic effect was the primary criterion of evaluation of antiviral activity. Reduction in cell and supernatant fluid virus titers was used as a secondary means of evaluation. The microplate system was adaptable for determining prophylactic, therapeutic, and inactivating effects against viruses. Mouse L-929 cells were used for the interferon induction studies, with vesicular stomatitis virus utilized as the indicator of interferon activity. Known active compounds evaluated in this microplate system had activity similar to that seen in macro in vitro systems.