Effects of nitrate treatment on a mixed species, oil field microbial biofilm
Effects of nitrate treatment on a mixed species, oil field microbial biofilm
复制标题
DOI：
10.1007/s10295-006-0095-2
复制
发表时间：
2006-06-01
影响因子：
3.4
通讯作者：
Vance, Ian
中科院分区：
文献类型：
--
作者：
Dunsmore, Braden;Youldon, James;Vance, Ian
在45 ℃下，在方形截面的玻璃毛细管流动池中生长油田采出水固有的细菌生物膜。最初，原位图像分析显微镜显示主要是球形细菌（细菌细胞的长宽比测量（l（c）：w（c））的平均值为1.1），而化学测量证实硫酸盐还原和硫化物产生。在100和80 mg/l的硝酸根离子添加后，在两个重复实验中分别观察到杆状细菌的优势（平均l（c）：w（c）= 2.8）。这与经处理的流动池中硝酸盐还原的发生相一致。有利的是，在添加硝酸盐之后没有观察到生物膜覆盖的显著增加。优势可培养硝酸盐还原菌为Marinessa aquaeolei。这里测量的l（c）：w（c）比与先前报道的该生物体的细胞尺寸一致。还从北海的不同油田中分离出几种Marinidae菌株，在这些油田中，硝酸盐处理已成功地用于处理储层酸化，这意味着该属可能在硝酸盐处理中发挥重要作用。
Biofilms of bacteria, indigenous to oil field produced water, were grown in square section, glass capillary flow cells at 45 degrees C. Initially, in situ image analysis microscopy revealed predominantly coccoid bacteria (length-to-width ratio measurements (l(c):w(c)) of bacterial cells gave a mean value of 1.1), while chemical measurements confirmed sulphate reduction and sulphide production. After nitrate ion addition at 100 and 80 mg/l, in the two repeat experiments respectively, the dominance of rod-shaped bacteria (mean l(c):w(c) = 2.8) was observed. This coincided with the occurrence of nitrate reduction in the treated flow cells. Beneficially, no significant increase in biofilm cover was observed after the addition of nitrate. The dominant culturable nitrate-reducing bacterium was Marinobacter aquaeolei. The l(c):w(c) ratio measured here concurs with previously reported cell dimensions for this organism. Several Marinobacter strains were also isolated from different oil fields in the North Sea where nitrate treatment has been applied to successfully treat reservoir souring, implying that this genus may play an important role in nitrate treatment.