Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies.
Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies.
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DOI：
10.1186/1746-1596-6-s1-s7
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发表时间：
2011-03-30
影响因子：
2.6
通讯作者：
Barbareschi M
中科院分区：
文献类型：
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作者：
Fasanella S;Leonardi E;Cantaloni C;Eccher C;Bazzanella I;Aldovini D;Bragantini E;Morelli L;Cuorvo LV;Ferro A;Gasperetti F;Berlanda G;Dalla Palma P;Barbareschi M
Ki 67标记指数（Ki 67 LI），即Ki 67免疫反应阳性细胞的百分比，是衡量肿瘤增殖的指标，在乳腺癌中具有重要的临床意义，其评价标准化极为重要。测试计算机辅助图像分析（CAIA）应用于完全数字化切片的有效性，评估其在常规实践中的可行性，并比较使用两种不同Ki 67单克隆抗体获得的结果。使用Aperio Scanscope Xs对315例连续乳腺癌进行Ki-67常规免疫染色（223例使用SP 6，92例使用MM 1抗体，之前由经验丰富的病理学家检查）。平均人Ki 67 LI值为36%± 14. SP 6和MM 1抗体的平均CAM Ki 67 LI值分别为31%± 19%和22% ± 18%。人类和CAIA评估在统计学上高度相关（Pearson：0.859，p<0.0001），尽管人类LI系统性地更高。对84例CAIA进行的观察者间变异研究表明，两种评价之间的相关性良好。我们的研究表明，a）CAIA可以很容易地在常规实践中采用，B）人类和CAIA Ki 67 LI高度相关，尽管人类LI系统性较高，c）使用不同评估方法和不同抗体的Ki 67 LI显示出重要差异截止值。
Ki67 labeling index (Ki67 LI), the percentage Ki67 immunoreactive cells, is a measure of tumor proliferation, with important clinical relevance in breast cancer, and it is extremely important to standardize its evaluation. To test the efficacy of computer assisted image analysis (CAIA) applied to completely digitized slides and to assess its feasibility in routine practice and compare the results obtained using two different Ki67 monoclonal antibodies. 315 consecutive breast cancer routinely immunostained for Ki-67 (223 with SP6 and 92 with MM1 antibodies previously examined by an experienced pathologist, have been re-evaluated using Aperio Scanscope Xs. Mean human Ki67 LI values were 36%± 14.% and 28% ± 18% respectively for SP6 and MM1 antibodies; mean CAM Ki67 LI values were 31%± 19% and 22% ± 18% respectively for SP6 and MM1. Human and CAIA evaluation are statistically highly correlated (Pearson: 0.859, p<0.0001), although human LI are systematically higher. An interobserver variation study on CAIA performed on 84 cases showed that the correlation between the two evaluations was linear to an excellent degree. Our study shows that a) CAIA can be easily adopted in routine practice, b) human and CAIA Ki67 LI are highly correlated, although human LI are systematically higher, c) Ki67 LI using different evaluation methods and different antibodies shows important differences in cut-off values.