Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens.
Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens.
复制标题
DOI：
10.1017/s1751731111001066
复制
发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
B. Zhang;X. Yang;Y. Guo;F. Long
中科院分区：
文献类型：
--
作者：
B. Zhang;X. Yang;Y. Guo;F. Long
本试验研究了日粮中添加脂类和酪酸梭菌对肉鸡循环脂质和脂类代谢相关基因转录水平的影响。选用160只日龄爱拔益加肉鸡，按2 × 2析因设计随机分为4个处理组，分别饲喂4种含2种脂肪源（大豆油或鱼油）和2种C.丁酸（0或5g/kg）持续6周。添加鱼油的肉鸡血清总胆固醇和低密度脂蛋白胆固醇浓度显著低于添加豆油的肉鸡（P < 0.05）。鱼油显著降低腹脂中肝脂肪酸合成酶和脂蛋白脂酶活性（P < 0.05）。相反，鱼油显著增加（P < 0.05）胸肌LPL活性和肝脏过氧化物酶体增殖物激活受体-α和LPL基因表达。C.丁酸能显著提高腹脂LPL活性和空肠粘膜肝型脂肪酸结合蛋白基因表达（P < 0.05）。本研究结果显示，饲以鱼油之肉鸡腹部脂肪减少，可能是由于肝脏脂肪酸催化剂增加及肝脏脂肪合成减少所致。结果表明，添加C.所述的丁酸盐可能是脂肪酸摄取增强的结果。
The effect of dietary lipids and Clostridium butyricum supplementation on circulating lipids and the transcription of selected genes involved in lipid metabolism were investigated in broiler chickens. One hundred and sixty-day-old broilers (Arbor Acres) were divided into four treatment groups in a 2 × 2 factorial arrangement and fed four diets with two lipid sources (soybean oil or fish oil) and two levels of C. butyricum (0 or 5 g/kg) for a period of 6 weeks. Serum concentrations of total cholesterol and low-density lipoprotein cholesterol were lower (P < 0.05) in broilers fed diets containing fish oil than in those fed diets containing soybean oil. Fish oil significantly reduced (P < 0.05) hepatic fatty acid synthase and lipoprotein lipase (LPL) activities in abdominal fat. In contrast, fish oil significantly increased (P < 0.05) breast muscle LPL activity and hepatic peroxisome proliferator-activated receptor-α and LPL gene expression. The addition of C. butyricum significantly increased (P < 0.05) LPL activity in abdominal fat and liver-type fatty acid-binding protein gene expression in jejunal mucosa. The results of this study indicated that the reduced abdominal fat in broilers fed fish oil as observed may be due to augmented hepatic fatty acid catabolism and lower hepatic fat synthesis. The increased intramuscular fat content in breast muscle of broilers fed C. butyricum as described may be the result of enhanced fatty acid uptake.