Prediction of plant microRNA targets
Prediction of plant microRNA targets
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DOI：
10.1016/s0092-8674(02)00863-2
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发表时间：
2002-08-23
期刊：
影响因子：
64.5
通讯作者：
Bartel, DP
中科院分区：
文献类型：
--
作者：
Rhoades, MW;Reinhart, BJ;Bartel, DP
我们通过识别具有近互补性的mRNAs来预测14个拟南芥microRNAs(MiRNAs)的调控靶标。预测靶点内的互补位点在水稻中是保守的。在49个预测靶点中，34个是参与发育模式或细胞分化的转录因子基因家族成员。植物miRNAs与其靶标之间的近乎完美的互补性表明，许多植物miRNAs的作用类似于小干扰RNA和直接切割mRNA。针对发育转录因子的研究表明，许多植物miRNAs在细胞分化过程中发挥作用，从子代细胞系中清除关键的调控转录。
We predict regulatory targets for 14 Arabidopsis microRNAs (miRNAs) by identifying mRNAs with near complementarity. Complementary sites within predicted targets are conserved in rice. Of the 49 predicted targets, 34 are members of transcription factor gene families involved in developmental patterning or cell differentiation. The near-perfect complementarity between plant miRNAs and their targets suggests that many plant miRNAs act similarly to small interfering RNAs and direct mRNA cleavage. The targeting of developmental transcription factors suggests that many plant miRNAs function during cellular differentiation to clear key regulatory transcripts from daughter cell lineages.