3 COMPLEMENTATION GROUPS IN COCKAYNE SYNDROME
3 COMPLEMENTATION GROUPS IN COCKAYNE SYNDROME
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DOI：
10.1016/0027-5107(82)90115-4
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
LEHMANN, AR
中科院分区：
文献类型：
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作者：
LEHMANN, AR
在16 Jm-2的UV照射后，非分裂正常细胞在24小时内恢复正常的RNA合成速率，而在来自Cockayne综合征（CS）供体的细胞中，RNA合成速率逐渐下降。将来自2个CS供体的混合群体的培养物与聚乙二醇融合;随后将其UV照射并在单核、双核和多核细胞中放射自显影测量RNA合成。与单核细胞相比，双核和多核细胞的RNA合成水平高，表明遗传互补。将11株CS菌株分为3个互补组：A组2株，B组8株，C组1株。C组中的菌株来源于也患有着色性干皮病（XP）的个体，并且是XP互补组B的唯一已知代表。
After 16 Jm-2 of UV-irradiation non-dividing normal cells recover normal rates of RNA synthesis within 24 h, whereas in cells from donors with Cockayne syndrome (CS) the rate of RNA synthesis gradually declines. Cultures of a mixed population from 2 CS donors were fused with polyethylene glycol; subsequently they were UV-irradiated and RNA synthesis was measured autoradiographically in mono-, bi- and multinuclear cells. Genetic complementation was indicated by high levels of RNA synthesis in bi- and multinuclear cells when compared with mononuclear cells. Using this assay, 11 CS strains were assigned to 3 complementation groups: 2 into group A, 8 into group B and 1 into group C. The strain in group C is derived from an individual who also had xeroderma pigmentosum (XP), and was the sole known representative of XP-complementation group B.