Effect of conjugated linoleic acids and omega-3 fatty acids with or without resistance training on muscle mass in high-fat diet-fed middle-aged mice
Effect of conjugated linoleic acids and omega-3 fatty acids with or without resistance training on muscle mass in high-fat diet-fed middle-aged mice
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DOI：
10.1113/ep086317
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发表时间：
2017-11-01
影响因子：
2.7
通讯作者：
Kim, Jeong-Su
中科院分区：
文献类型：
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作者：
Lee, Sang-Rok;Khamoui, Andy V.;Kim, Jeong-Su
新发现 这项研究的中心问题是什么？本研究考察了服用共轭亚油酸/omega-3 脂肪酸 20 周，并进行或不进行程序性抗阻运动训练，对高脂饮食中年小鼠的身体成分、骨骼肌特性和功能能力的影响。主要发现是什么及其重要性？长期每日服用共轭亚油酸/omega-3脂肪酸并进行抗阻运动训练，有助于抑制中年小鼠在慢性高脂饮食诱导的分解代谢过程中的脂肪增加，减轻肌源能力和感觉运动功能的损失，并降低组织炎症。本研究调查了单独或联合抗阻运动训练（RET）联合服用共轭亚油酸（CLA）/omega-3脂肪酸（n-3）20周对骨骼肌的影响食用高脂肪饮食（HFD）的中年小鼠。九个月大的 C57BL/6 小鼠被随机分为四个实验组（H，高脂饮食；HE，H+RET；HCN，H+CLA/n-3；和 HECN，H+CLA/n3+RET）。在干预前后评估身体成分和功能能力。 14个月大时收集肌肉组织。使用方差分析，显着性设置为 P0.05。 H (+74%)、HE (+142%) 和 HECN (+43%) 中的脂肪量显着增加，但 HCN 中则没有。 H 和 HCN 组的肌肉湿重显着低于 HE 和 HECN 组。 H (-15%) 和 HCN (-17%) 的握力显着下降，而仅 H (-11%) 的感觉运动功能显着下降。 HECN 在力量 (+22%) 和感觉运动协调性 (+17%) 方面表现出改善。与 H 相比，HE (-39%)、HCN (-24%) 和 HECN (-21%) 中的肌肉肿瘤坏死因子 mRNA 表达分别显着较低。 H 和 HCN 中的平均肌纤维横截面积明显低于 HE 和 HECN 中。 H 显示出明显低于所有其他组的卫星细胞丰度和肌核数量。我们的研究结果表明，长期每日摄入 CLA/n-3 并进行阻力训练可改善感觉运动功能，改善脂肪增加并防止生肌能力丧失，同时降低慢性 HFD 期间肿瘤坏死因子的表达。
New Findings What is the central question of this study? This study examined the effects of 20weeks of administration of conjugated linoleic acids/omega-3 fatty acids with or without programed resistance exercise training on body composition, skeletal muscle properties and functional capacity in middle-aged mice fed a high-fat diet.What is the main finding and its importance? Chronic daily administration of conjugated linoleic acids/omega-3 fatty acids with resistance exercise training can help to blunt fat gain, alleviate loss of myogenic capacity and sensorimotor function and lower tissue inflammation in middle-aged mice during chronic high-fat diet-induced catabolism.This study investigated the effects of 20weeks of combined conjugated linoleic acid (CLA)/omega-3 fatty acid (n-3) administration independently or combined with resistance exercise training (RET) on skeletal muscle in middle-aged mice consuming a high-fat diet (HFD). Nine-month-old C57BL/6 mice were randomly assigned into four experimental groups (H, high-fat diet; HE, H+RET; HCN, H+CLA/n-3; and HECN, H+CLA/n3+RET). Body composition and functional capacity were assessed pre- and post-intervention. Muscle tissues were collected at 14months of age. ANOVA was used, with significance set at P0.05. Fat mass significantly increased in H (+74%), HE (+142%) and HECN (+43%) but not in HCN. Muscle wet weights were significantly lower in H and HCN than in HE and HECN. Grip strength substantially declined in H (-15%) and HCN (-17%), whereas sensorimotor function significantly declined only in H (-11%). HECN exhibited improvement in strength (+22%) and sensorimotor coordination (+17%). In comparison to H, muscle tumour necrosis factor- mRNA expression was significantly lower in HE (-39%), HCN (-24%) and HECN (-21%), respectively. Mean myofibre cross-sectional areas were markedly lower in H and HCN than in HE and HECN. H showed significantly lower satellite cell abundance and numbers of myonuclei than all other groups. Our findings suggest that long-term daily CLA/n-3 intake with resistance training improved sensorimotor function, ameliorated fat gain and prevented loss of myogenic capacity while lowering tumour necrosis factor- expression during chronic HFD.