IDENTIFICATION OF A NONNEURONAL ISOFORM OF SYNAPTOTAGMIN
IDENTIFICATION OF A NONNEURONAL ISOFORM OF SYNAPTOTAGMIN
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DOI：
10.1073/pnas.92.13.5895
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发表时间：
1995-06-20
影响因子：
11.1
通讯作者：
BIRNBAUM, MJ
中科院分区：
文献类型：
--
作者：
HUDSON, AW;BIRNBAUM, MJ
突触素仅存在于神经内分泌或外分泌组织中，参与调节分泌囊泡与质膜的融合。本论文描述了一种突触素亚型(synaptopagmin-5)，它与突触素-1和-2有49%的氨基酸同源性。突触素-5mRNA在大鼠肾脏、脂肪组织、肺和心脏中表达，在脑和PC12细胞中表达水平较高。针对突触素-5亚型的抗血清识别一种约50 kDa的蛋白质，这种蛋白质在脑突触小泡中的含量是在整个脑膜中的6倍。
Synaptotagmins, which have been found exclusively in neuroendocrine or exocrine tissues, have been implicated in the regulation of secretory vesicle fusion with the plasma membrane. The present paper describes a synaptotagmin isoform (synaptotagmin-5) which exhibits 49% amino acid identity to synaptotagmin-1 and -2. Synaptotagmin-5 mRNA is expressed in rat kidney, adipose tissue, lung, and heart, as well as at higher levels in brain and PC12 cells. Antiserum specific for the synaptotagmin-5 isoform recognizes a protein of about 50 kDa which is about 6-fold more abundant in brain synaptic vesicles than in whole brain membranes.