Resetting of circadian time peripheral tissues by glucocorticoid signaling
Resetting of circadian time peripheral tissues by glucocorticoid signaling
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DOI：
10.1126/science.289.5488.2344
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发表时间：
2000-09-29
期刊：
影响因子：
56.9
通讯作者：
Schibler, U
中科院分区：
文献类型：
--
作者：
Balsalobre, A;Brown, SA;Schibler, U
在哺乳动物中，昼夜节律振荡器不仅存在于大脑的视交叉上核中，也存在于大多数周围组织中。在这里，我们发现糖皮质激素类似物地塞米松诱导培养的大鼠1号成纤维细胞的昼夜节律基因表达，并短暂改变肝脏、肾脏和心脏的昼夜节律基因表达的阶段。地塞米松不影响视交叉上核神经元环基因的表达。这使我们能够建立一个明显的相移响应曲线，专门为外围时钟在完整的动物。与中央时钟相反，外周组织中的昼夜节律振荡器似乎对全天的相位重置保持反应。
In mammals, circadian oscillators reside not only in the suprachiasmatic nucleus of the brain, which harbors the central pacemaker, but also in most peripheral tissues. Here, we show that the glucocorticoid hormone analog dexamethasone induces circadian gene expression in cultured rat-1 fibroblasts and transiently changes the phase of circadian gene expression in liver, kidney, and heart. However, dexamethasone does not affect cyclic gene expression in neurons of the suprachiasmatic nucleus. This enabled us to establish an apparent phase-shift response curve specifically for peripheral clocks in intact animals. In contrast to the central clock, circadian oscillators in peripheral tissues appear to remain responsive to phase resetting throughout the day.