Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures
Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures
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DOI：
10.1016/j.fertnstert.2006.06.059
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发表时间：
2007-03-01
影响因子：
6.7
通讯作者：
Roccheri, Maria C.
中科院分区：
文献类型：
--
作者：
Ruvolo, Giovanni;Bosco, Liana;Roccheri, Maria C.
目的：探讨重组(r-)促黄体生成素(RFSH)在低反应者体外受精(IVF)中的应用效果。以卵丘细胞凋亡率作为卵母细胞质量的指标。设计：比较促黄体生成素和促卵泡刺激素与单纯促卵泡刺激素治疗患者卵丘细胞DNA片段率和caspase-3活性的差异(对照组)。地点：体外受精(IVF)实验室。患者(S)：40例接受辅助受精的患者，在周期的第3天开始接受促性腺激素释放激素激动剂或r-卵泡刺激素治疗(对照)。从促性腺激素刺激第8天开始，给予r-LH150IU/d。干预(S)：末端脱氧核苷酸转移酶介导的地高辛-脱氧尿苷-三磷酸(DUTP)缺口末端标记(TUNEL)法和抗caspase-3裂解免疫分析法检测人卵丘细胞的凋亡。主要观察指标(S)：经r-LHDNA处理的卵丘细胞与来自对照组的卵丘细胞在DNA碎裂率方面存在差异。结果(S)：两组患者的r-FSH总量和每例患者的获卵数均无差异。对照组未成熟卵母细胞数和血清E-2峰值显著增加。重组人促黄体素组移植胚胎数显著高于对照组。促黄体生成素组妊娠率和着床率较高，但无统计学意义。对照组卵丘细胞凋亡率明显高于r-LH组。结论：补充r-LH值可提高卵丘细胞染色质的质量，参与卵母细胞成熟的调控。(Fertil Steril(R)2007；87：542-6.(C)2007年，由美国生殖医学会提供。)
Objective: To investigate the effects of recombinant (r-) LH supplementation in "low responder" patients undergoing ovarian stimulation with r-FSH for an IVF program. The apoptosis rate in cumulus cells was used as an indicator of oocyte quality.Design: Comparison of the rate of DNA fragmentation and caspase-3 activity in cumulus cells in women stimulated with r-LH and r-FSH, versus patients treated with r-FSH alone (control).Setting: In vitro fertilization (IVF) laboratory.Patient(s): Forty patients undergoing assisted fertilization programs treated with a GnRH agonist, or r-FSH treatment begun on day 3 of the cycle (control). In the r-LH group, from day 8 of gonadotropin stimulation, 150 IU per day of r-LH were administered.Intervention(S): Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine-triphosphate (dUTP) nick-end labeling (TUNEL) assay, and anti-caspase-3 cleaved immunoassay, to detect apoptosis in human cumulus cells.Main Outcome Measure(s): Difference in DNA fragmentation rate between cumulus cells derived from r-LH treatment and cumulus cells derived from control patients.Result(S): No differences were observed between the two groups in the total amount of r-FSH administered and in the number of retrieved oocytes per patient. A statistically significant increase in the number of immature oocytes and in the E-2 serum peak was observed in the control group. The number of transferred embryos was significantly higher in the r-LH group. Pregnancy and implantation rates were higher in the r-LH group, but without statistical significance. The apoptosis rate in cumulus cells was higher in the control group than in the r-LH group.Conclusion(s): This study suggests that supplementation with r-LH improves the chromatin quality of cumulus cells involved in the control of oocyte maturation. (Fertil Steril (R) 2007;87:542-6. (c) 2007 by American Society for Reproductive Medicine.)