Role of oxygen tension during cartilage formation by periosteum
Role of oxygen tension during cartilage formation by periosteum
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DOI：
10.1002/jor.1100150509
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发表时间：
1997-09-01
影响因子：
2.8
通讯作者：
Commisso, CN
中科院分区：
文献类型：
--
作者：
O'Driscoll, SW;Fitzsimmons, JS;Commisso, CN
组织工程使软骨再生成为可能，但需要优化培养条件。氧张力对软骨代谢的影响在文献中是有争议的，Ne没有找到关于生长新软骨（neochondrogenesis）的最佳氧浓度的详细信息。骨膜细胞和组织可用于体内和体外培养软骨。在本研究中，我们使用标准的骨膜器官培养模型，发现骨膜外植体的软骨形成受到环境氧浓度的影响。取30只2月龄新西兰大白兔480块骨膜外植体，在琼脂糖悬浮液中不同氧浓度（1-90%）培养6周。软骨形成，通过组织形态学和定量胶原分型分析，在12-15%氧下最大。在12-45%的范围内，软骨形成无显著差异。在非常高（90%）和非常低（1-5%）的氧浓度下，软骨和ii型胶原的形成受到抑制。然而，与一些人认为的相反，软骨形成在有氧条件下是最大的。如果这对于骨膜植入物以外的系统是正确的，那么它将对体外培养软骨具有重要意义。
Tissue engineering makes regeneration of cartilage possible but requires optimization of culture conditions. The effects of oxygen tension on cartilage metabolism are controversial in the literature, and Ne could find no information detailing the optimal oxygen concentration for growing new cartilage (neochondrogenesis). Periosteal cells and tissues can be used to grow cartilage in vivo and in vitro. In this study, using a standard periosteal organ culture model, we found that cartilage formation by periosteal explants is affected by the ambient oxygen concentrations. A total of 480 periosteal explants from 30 2-month-old New Zealand White rabbits were cultured in agarose suspension at different oxygen concentrations (1-90%) for 6 weeks. Chondrogenesis, which was analyzed by histomorphometry and quantitative collagen typing, was maximal at 12-15% oxygen. There were no significant differences in chondrogenesis in the range of 12-45%. There was inhibition of cartilage and type-II collagen formation at very high (90%) and very low (1-5%) oxygen concentrations. However, contrary to what some have thought, chondrogenesis is maximal under aerobic conditions. If this is true for systems other than periosteal implants, it would have important implications for growing cartilage in vitro.