MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism
MAL62 Overexpression Enhances Freezing Tolerance of Baker's Yeast in Lean Dough by Enhancing Tps1 Activity and Maltose Metabolism
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MAL62 过表达通过增强 Tps1 活性和麦芽糖代谢来增强瘦面团中面包酵母的冷冻耐受性
DOI：
10.1021/acs.jafc.9b03790
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发表时间：
2019
影响因子：
6.1
通讯作者：
Huang Liang
中科院分区：
文献类型：
--
作者：
Sun Xi;Zhang Jun;Fan Zhi Hua;Xiao Ping;Liu Shan Na;Li Rui Peng;Zhu Wen Bi;Huang Liang
海藻糖在面包酵母对冷冻胁迫的应答中起着重要作用，MAL62基因是腺苷二磷酸葡萄糖依赖的海藻糖合成途径中的一个基因，可以提高海藻糖的含量。然而，MAL62相关的海藻糖合成与传统尿苷二磷酸葡萄糖依赖的海藻糖合成之间的差异尚不清楚，与TPS 1过表达相比，MAL62过表达对细胞内海藻糖合成的促进作用较小。然而，MAL62过表达引起发酵前海藻糖的合成，增强麦芽糖代谢，并在冷冻后对细胞活力有类似的影响。此外，在NTH1缺失背景下，MAL62和TPS1的过表达进一步增强了耐冻性，提高了发酵能力。我们的研究结果表明，MAL62过表达的抗冻性的增强可能涉及多个途径，而不是简单地增加海藻糖的合成。研究结果揭示了麦芽糖代谢与耐冷冻性之间关系的宝贵见解，并可能有助于开发更好的酵母菌株来增强冷冻面团的发酵特性。
Trehalose plays a crucial role in response to freezing stress in baker’s yeast.MAL62, a gene involved in the adenosine diphosphoglucose-dependent trehalose synthesis pathway, can increase trehalose content. However, the difference betweenMAL62-related trehalose synthesis and traditional uridine diphosphoglucose-dependent trehalose synthesis is not well-understood.MAL62overexpression showed less effect in enhancing intracellular trehalose compared toTPS1overexpression. However,MAL62overexpression elicited trehalose synthesis before fermentation with enhanced maltose metabolism and had a similar effect on cell viability after freezing. Furthermore,MAL62andTPS1overexpression in theNTH1deletion background further strengthened freezing tolerance and improved leavening ability. Our results suggest that the enhancement in freezing tolerance byMAL62overexpression may involve multiple pathways rather than simply enhancing trehalose synthesis. The results reveal valuable insights into the relationship between maltose metabolism and freezing tolerance and may help to develop better yeast strains for enhancing fermentation characteristics of frozen dough.