Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells
Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells
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DOI：
10.1007/s10439-010-0115-2
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Asahina, Izumi
中科院分区：
文献类型：
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作者：
Ikeda, Hideyoshi;Sumita, Yoshinori;Asahina, Izumi
尚未建立一种从培养的牙科间充质细胞诱导骨形成的可靠方法。在这项研究中，设计了一种使用外源骨形态发生蛋白 2 (BMP2) 在体内从培养的人牙髓和牙周膜衍生细胞（分别为 DPC 和 PDLC）生成骨组织的方法。在含有地塞米松、β-甘油磷酸盐和抗坏血酸（成骨培养基）的培养基中，DPC 和 PDLC 表现出增强的碱性磷酸酶（ALP）活性和钙化结节形成。然而，在成骨培养基中添加重组人骨形态发生蛋白 2 (rhBMP2) 显着增加了 ALP 活性和体外钙化，高于单独使用成骨培养基观察到的增加。 rhBMP2 还显着上调在成骨培养基中培养的两种细胞类型中骨钙素、骨桥蛋白和牙本质基质蛋白 1 mRNA 的表达。最后，当细胞暴露于成骨培养基中的 rhBMP2 时，我们在体内检测到显着的骨样组织形成。相反，单独使用成骨培养基或rhBMP2处理不能诱导丰富的矿化组织形成。我们在此提出，在成骨培养基中用 rhBMP2 处理可以使牙间充质组织成为骨组织工程非常有用的细胞来源。此外，DPC 和 PDLC 均表现出相似且显着的骨诱导能力。
A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue in vivo from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, beta-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and in vitro calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of osteocalcin, osteopontin, and dentin matrix protein 1 mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation in vivo when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.