Stress-activated Genomic Expression Changes Serve a Preparative Role for Impending Stress in Yeast
Stress-activated Genomic Expression Changes Serve a Preparative Role for Impending Stress in Yeast
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DOI：
10.1091/mbc.e07-07-0680
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Gasch, Audrey P.
中科院分区：
文献类型：
--
作者：
Berry, David B.;Gasch, Audrey P.
酵母细胞通过调节条件特异性基因表达变化和对多种压力产生共同反应来应对压力，称为环境压力反应（ESR）。Giaever等人先前发现，在急性应激反应中表达变化的基因与生存所需的基因之间存在较差的相关性，这引发了对应激激活基因表达作用的质疑。在这里，我们表明，由单一剂量的压力触发的基因表达变化并不是生存刺激所必需的，而是对未来压力的保护作用。我们的特点是增加抵抗严重的压力在酵母预暴露于温和的压力。这种获得性抗逆性依赖于轻度胁迫处理期间的蛋白质合成，并需要调节许多ESR基因诱导的“一般应激”转录因子Msn2p和/或Msn4p。然而，无论蛋白合成还是Msn2/4p都不是单剂量胁迫基础耐受性所必需的，尽管每种条件都会引发实质性的表达变化。利用微阵列，我们发现Msn2p和Msn4p在基因表达调控中发挥非冗余和条件特异性作用，反对通用的一般应激功能。这项工作强调了条件特异性反应在获得性应激抵抗中的重要性，并为ESR的作用提供了新的见解。
Yeast cells respond to stress by mediating condition-specific gene expression changes and by mounting a common response to many stresses, called the environmental stress response (ESR). Giaever et al. previously revealed poor correlation between genes whose expression changes in response to acute stress and genes required to survive that stress, raising question about the role of stress-activated gene expression. Here we show that gene expression changes triggered by a single dose of stress are not required to survive that stimulus but rather serve a protective role against future stress. We characterized the increased resistance to severe stress in yeast preexposed to mild stress. This acquired stress resistance is dependent on protein synthesis during mild-stress treatment and requires the "general-stress" transcription factors Msn2p and/or Msn4p that regulate induction of many ESR genes. However, neither protein synthesis nor Msn2/4p is required for basal tolerance of a single dose of stress, despite the substantial expression changes triggered by each condition. Using microarrays, we show that Msn2p and Msn4p play nonredundant and condition-specific roles in gene-expression regulation, arguing against a generic general-stress function. This work highlights the importance of condition-specific responses in acquired stress resistance and provides new insights into the role of the ESR.