Impact of exudative diathesis induced by selenium deficiency on LncRNAs and their roles in the oxidative reduction process in broiler chick veins.
Impact of exudative diathesis induced by selenium deficiency on LncRNAs and their roles in the oxidative reduction process in broiler chick veins.
复制标题
缺硒引起的渗出素质对 LncRNA 的影响及其在肉鸡静脉氧化还原过程中的作用。
DOI：
10.18632/oncotarget.14971
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发表时间：
2017-03-28
期刊：
影响因子：
--
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Cao C;Fan R;Zhao J;Zhao X;Yang J;Zhang Z;Xu S
缺硒可导致肉鸡渗出性素质(ED)，这种损伤与氧化损伤密切相关。在体内，长非编码RNA(LncRNA)可以调节氧化还原状态。本研究的目的是阐明lncRNA在肉鸡静脉中的表达谱，筛选和验证与ED氧化损伤相关的lncRNA。本研究建立了缺硒致勃起功能障碍动物模型，并对正常和勃起功能障碍组织中lncRNAs的表达和特性进行了研究。利用Illumina Hi-Seq4000平台在6个文库中构建了15412个LncRNA(包括8052个新的LncRNAs)。筛选出635个差异表达的LncRNAs(上调折叠改变1.5，下调折叠改变0.67，差异表达的LncRNAs)。在lncRNAs靶基因上的基因本体丰富表明氧化还原过程是重要的。本研究还定义并验证了与氧化还原过程相关的23个lncRNAs中的19个靶mRNAs。体内和体外实验也证明这23个lncRNAs可以参与氧化还原过程。本研究首次报道了lncRNAs在肉鸡静脉中的表达谱，证实了23个lncRNAs参与了ED的静脉氧化损伤。
Selenium deficiency may induce exudative diathesis (ED) in broiler chick, and this damage is closely related to oxidative damage. Long noncoding RNA (LncRNA) can regulate the redox state in vivo. The aim of the present study was to clarify the LncRNA expression profile in broiler veins and filter and verify the LncRNAs related to oxidative damage of ED. This study established an ED model induced by selenium deficiency and presented the expression and characterization of LncRNAs in normal and ED samples. A total of 15412 LncRNAs (including 8052 novel LncRNAs) were generated in six cDNA libraries using the Illumina Hi-Seq 4000 platform. 635 distinct changes in LncRNAs (up-regulated fold change > 1.5, down-regulated fold change < 0.67 and differentially expressed LncRNAs) were filtered. Gene ontology enrichment on LncRNAs target genes showed that the oxidative reduction process was important. This study also defined and verified 19 target mRNAs of 23 LncRNAs related to the oxidative reduction process. The in vivo and vitro experiments also demonstrated these 23 LncRNAs can participate in the oxidative reduction process. This study presents LncRNAs expression profile in broiler chick veins for the first time and confirmed 23 LncRNAs involving in the vein oxidative damage in ED.