Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases
Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases
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利用大肠杆菌在加压 H 2 和 CO 2 气体下生物基生产甲酸盐
DOI：
10.1101/2021.01.06.425572
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Roger M
中科院分区：
文献类型：
--
作者：
Roger M
大肠杆菌是一种革兰氏阴性细菌，是生物技术的主力。该生物体在厌氧条件下自然地进行混合酸发酵，其中它合成甲酸氢解酶（FHL-1）。该酶的生理作用是将甲酸盐还原成H2和CO2。然而，已经观察到该酶在正确的条件下催化CO2的氢化，因此如果它可以作为氢依赖性CO2还原酶（HDCR）利用，则它在生物基碳捕获和储存中具有可能性。在本研究中，E.大肠杆菌宿主菌在加压间歇式生物反应器中利用H2和CO2连续生产甲酸。在FHL-1中掺入钨代替钼有助于对酶施加一定程度的催化偏倚。这项工作表明，它是可能的耦合细胞生长的同时，单向甲酸生产二氧化碳和开发一个过程中的增长下加压gas.IMPORTANCEGreenhouse气体排放，包括废弃的二氧化碳，是导致全球气候变化。需要一篮子解决方案来稳步减少排放，其中一种方法是生物基碳捕获和储存。在这里，我们介绍了我们关于利用新型生物解决方案进行碳捕获的最新工作。大肠杆菌甲酸氢裂解酶（FHL-1）的工程组成型表达。建立了加压H2和CO2气体下的厌氧生长，并由此产生甲酸水溶液。将钨掺入到酶中代替钼被证明是有用的，作为氢依赖性CO2还原酶（HDCR）平衡FHL-1。
Escherichia coli is a Gram-negative bacterium that is a workhorse for biotechnology. The organism naturally performs a mixed-acid fermentation under anaerobic conditions where it synthesizes formate hydrogenlyase (FHL-1). The physiological role of the enzyme is the disproportionation of formate into H2and CO2. However, the enzyme has been observed to catalyze hydrogenation of CO2given the correct conditions, and so it has possibilities in bio-based carbon capture and storage if it can be harnessed as a hydrogen-dependent CO2reductase (HDCR). In this study, an E. coli host strain was engineered for the continuous production of formic acid from H2and CO2during bacterial growth in a pressurized batch bioreactor. Incorporation of tungsten, in place of molybdenum, in FHL-1 helped to impose a degree of catalytic bias on the enzyme. This work demonstrates that it is possible to couple cell growth to simultaneous, unidirectional formate production from carbon dioxide and develops a process for growth under pressurized gases.IMPORTANCEGreenhouse gas emissions, including waste carbon dioxide, are contributing to global climate change. A basket of solutions is needed to steadily reduce emissions, and one approach is bio-based carbon capture and storage. Here, we present our latest work on harnessing a novel biological solution for carbon capture. The Escherichia coli formate hydrogenlyase (FHL-1) was engineered to be constitutively expressed. Anaerobic growth under pressurized H2and CO2gases was established, and aqueous formic acid was produced as a result. Incorporation of tungsten into the enzyme in place of molybdenum proved useful in poising FHL-1 as a hydrogen-dependent CO2reductase (HDCR).