Two new species of the genus Micromonospora:: Micromonospora chokoriensis sp nov and Micromonospora coxensis sp nov., isolated from sandy soil
Two new species of the genus Micromonospora:: Micromonospora chokoriensis sp nov and Micromonospora coxensis sp nov., isolated from sandy soil
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DOI：
10.2323/jgam.53.29
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发表时间：
2007-02-01
影响因子：
1.2
通讯作者：
Kudo, Takuji
中科院分区：
文献类型：
--
作者：
Ara, Ismet;Kudo, Takuji
从孟加拉国考克斯巴扎尔的乔科里亚（Chokoria）的桑迪土壤中分离到两株放线菌2-19（6）（T）和2-30-B（28）（T），它们产生单个的、不运动的节瘤至疣状孢子表面。进行了多相研究，以建立这些菌株的分类地位。这些菌株的形态学和化学分类学特征与小单孢菌属一致。系统发育分析表明，这些菌株应归属于小单孢菌属（Micromonospora）。菌株2-19（6）（T）的16 S rDNA序列与M. mirobrigensis（98.9%）和M. 2-30-B（28）（T）与M.紫色产色菌（99.4%）、M. salophytica（99.3%）和M.橙花属（aurantiaca）占99.2%。此外，结合DNA-DNA杂交结果和一些差异的生理生化特性表明，这些菌株是区别于最近的亲缘关系。因此，这些菌株代表了两个新的物种，建议将其命名为Micromonospora chokoriensis sp. nov.和Micromonospora coxensis sp. nov.。模式菌株为2-19（6）（T）（=JCM 13247（T）=MTCC 8535（T））和2-30-B（28）（T）（=JCM 13248（T）=MTCC 8093（T））。
Two actinomycete strains, 2-19(6)(T) and 2-30-b(28)(T), which produced single, non-motile noduler to warty spore surfaces, were isolated from sandy soil in Chokoria, Cox's Bazar, Bangladesh. A polyphasic study was carried out to establish the taxonomic position of these strains. Morphological and chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora. Phylogenetic analysis using 16S rDNA sequences indicated that these strains should be classified in the genus Micromonospora. The 16S rDNA sequence of strain 2-19(6)(T) showed closest similarity to the type strains of M. mirobrigensis (98.9%) and M. carbonacea (98.8%), and the strain 2-30-b(28)(T) to the type strains of M. purpureochromogenes (99.4%), M. halophytica (99.3%) and M. aurantiaca (99.2%). Furthermore, a combination of DNA-DNA hybridization results and some differential physiological and biochemical properties indicated that these strains were distinguished from the phylogenetically closest relatives. These strains therefore represent two novel species, for which the name Micromonospora chokoriensis sp. nov. and Micromonospora coxensis sp. nov. are proposed. The type strains are 2-19(6)(T) (=JCM 13247(T)=MTCC 8535(T)) and 2-30-b(28)(T) (=JCM 13248(T)=MTCC 8093(T)).