The influence of oxygen toxicity on yeast mother cell-specific aging
The influence of oxygen toxicity on yeast mother cell-specific aging
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DOI：
10.1016/s0531-5565(99)00087-x
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发表时间：
2000-02-01
影响因子：
3.9
通讯作者：
Breitenbach, M
中科院分区：
文献类型：
--
作者：
Nestelbacher, R;Laun, P;Breitenbach, M
研究了删除过氧化氢酶基因和增加氧气以及百草枯（一种Dro-氧化剂）对酵母母细胞复制寿命的影响，以检验所谓的老化氧理论。这在高等生物中已得到很好的证实，但尚未在单细胞酵母模型系统中进行广泛的测试。寿命测定在环境空气中或在受控气氛（55%氧气）中进行，并使用一种或两种酵母过氧化氢酶缺失的等基因系列菌株，并与野生型进行比较。在没有细胞过氧化氢酶的情况下，增加的氧气导致寿命的显着下降，可以通过向酵母生长培养基中加入1 mM GSH（一种生理抗氧化剂）完全逆转。在第二个不相关的菌株中，效果相似，尽管当氧气增加时，即使是野生型也显示出寿命缩短。该效应可以通过添加细胞外GSH再次得到补偿。我们的研究结果表明，操纵活性氧的解毒对酵母老化有深远的影响。这些研究结果进行了讨论，根据最近的结果有关的氧毒性在老化过程中的高等生物。(C)2000 Elsevier Science Inc. All rights reserved.
The effect of deleting both catalase genes and of increased oxygen as well as paraquat (a Dro-oxidant) on the replicative life span of yeast mother cells has been investigated to test the so-called oxygen theory of aging. This is well established in higher organisms,but has not been extensively tested in the unicellular yeast model system. Life span determinations were performed in ambient air or in a controlled atmosphere (55% oxygen) and an isogenic series of strains deleted for one or both yeast catalases was used and compared with wild type. In the absence of cellular catalase, increased oxygen caused a marked decrease in life span that could be completely reversed by adding 1 mM GSH, a physiological antioxidant, to the yeast growth medium. In a second unrelated strain, the effects were similar although even the wild type showed a decrease in life span when oxygen was increased. The effect could again be compensated by addition of extracellular GSH. Our results show that manipulating the detoxification of reactive oxygen species has a profound effect on yeast aging. These findings are discussed in the light of recent results relating to oxygen toxicity in the aging process of higher organisms. (C) 2000 Elsevier Science Inc. All rights reserved.